Evaluation of the performance of serum miRNAs as normalizers in microRNA studies focused on cardiovascular disease

Journal of Thoracic Disease
Xiaoyan WangYunzeng Zou

Abstract

Few study has been done to evaluate the stability and superiority of normalizers for serum microRNA (miRNA) study in cardiovascular disease. Therefore, the aim of this study is to assess the suitability of several common normalizers (miR-16, SNOU6, 5S, miR-19b, miR-24, miR-15b, let 7i) in cardiovascular disease. We evaluated the stability of the seven circulating miRNAs as reference genes in the blood samples from patients with cardiovascular disease [heart failure (HF) and hypertension] and healthy people. Stability was quantified by combining BestKeeper, NormFinder and comparative delta Cq analysis. A total of 62 subjects were included in this study, of which 25 patients were with HF, 10 patients were with hypertension, and 27 were healthy people. The analysis from both BestKeeper and comparative delta ct analysis demonstrated that let-7i and miR-16 showed the best performance [the standard deviations (SD) in BestKeeper for let-7i and miR-16 were 0.60 and 0.72, and the mean SD in comparative delta ct analysis for let-7i and miR-16 were 1.79 and 1.82, respectively], while SNOU6 and 5S had the highest variability. In NormFinder analysis, miR-15 show best stability (ρ=0.029), followed by miR-19b (ρ=0.037), let-7i (ρ=0.064), SNOU...Continue Reading

Citations

Aug 9, 2020·Diabetes, Metabolic Syndrome and Obesity : Targets and Therapy·Shereen AbdelghaffarYasmine Elshiwy
May 16, 2019·Cellular and Molecular Life Sciences : CMLS·Soheil MadadiMeysam Soleimani
May 14, 2020·Clinical and Experimental Medicine·Ilgiz GareevShiguang Zhao
Mar 12, 2021·Frontiers in Endocrinology·Sharmilee VetrivelAnna Riester
Sep 12, 2021·Advances in Medical Sciences·Ewa Moric-JaniszewskaMałgorzata Kapral
Nov 21, 2021·Pediatric Research·Cristina Garcia-BeltranLourdes Ibáñez

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