Evaluation of the potential of doxorubicin loaded microbubbles as a theranostic modality using a murine tumor model

Acta Biomaterialia
Rodi AbdalkaderMitsuru Hashida

Abstract

In this study, a novel phospholipid-based microbubble formulation containing doxorubicin and perfluoropropane gas (DLMB) was developed. The DLMBs were prepared by mechanical agitation of a phospholipid dispersion in the presence of perfluoropropane (PFP) gas. An anionic phospholipid, distearoyl phosphatidylglycerol (DSPG) was selected to load doxorubicin in the microbubbles by means of electrostatic interaction. The particle size, zeta potential, echogenicity and stability of the DLMBs were measured. Drug loading was ⩾ 92%. The potential of the DLMBs for use as a theranostic modality was evaluated in tumor bearing mice. Gas chromatography analysis of PFP showed significant enhancement of PFP retention when doxorubicin was used at concentrations of 10-82% equivalent to DSPG. The inhibitory effects on the proliferation of B16BL6 melanoma murine cells in vitro were enhanced using a combination of ultrasound (US) irradiation and DLMBs. Moreover, in vivo DLMBs in combination with (US) irradiation significantly inhibited the growth of B16BL6 melanoma tumor in mice. Additionally, US echo imaging showed high contrast enhancement of the DLMBs in the tumor vasculature. These results suggest that DLMBs could serve as US triggered carriers...Continue Reading

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Citations

Jan 27, 2016·Journal of Drug Targeting·Xing HuaQinghai Chen
Aug 27, 2016·Carbohydrate Polymers·Guilherme F PichethRilton Alves de Freitas
Nov 5, 2016·IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control·Helen MulvanaEleanor Stride
Feb 5, 2019·Medicine, Conflict, and Survival·Elizabeth Waterston, Frank Boulton
Jul 5, 2017·Chemical & Pharmaceutical Bulletin·Masayori HagimoriShigeru Kawakami
Dec 23, 2017·Advanced Materials·Chunxiao LiPeng Huang
Sep 27, 2019·Biological & Pharmaceutical Bulletin·Rodi AbdalkaderMitsuru Hashida
Jul 5, 2017·Chemical & Pharmaceutical Bulletin·Shintaro Fumoto, Koyo Nishida
Jun 11, 2021·Cell & Bioscience·Asma AkbarMujib Ullah
May 19, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Jonah S HarmonJoseph L Bull
Jul 14, 2020·Advanced Drug Delivery Reviews·Daiki OmataKazuo Maruyama

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