Evaluation of the rat bladder-derived relaxant factor by coaxial bioassay system

European Journal of Pharmacology
Turgut Emrah Bozkurt, I Sahin-Erdemli

Abstract

The release of bladder-derived relaxant factor in a coaxial bioassay system and the effects of reactive oxygen species were studied. After precontraction with phenylephrine (10(-6)-3x10(-6)) or 50 mM K+, acetylcholine (10(-8)-10(-3) M) induced relaxation in rat anococcygeus muscle mounted within rat bladder in a tissue bath. This relaxation was not altered by the removal of the urothelium or incubation with tetrodotoxin (10(-6) M). However, bupivacaine (10(-4) M) and lidocaine (3 x 10(-4) M) inhibited this response after raising the pH of the nutrient solution to 7.8, and oxybuprocaine (10(-4) M) exerted inhibitory effect at both physiological pH (7.4) and at pH 7.8. Exposure to electrolysis-generated reactive oxygen species or incubation with hydrogen peroxide and pyrogallol did not alter the acetylcholine response. Present results indicate that the bladder-derived relaxant factor does not behave like endothelium-derived hyperpolarizing factor, but its release may be associated with tetrodotoxin-resistant Na+ channels, which are probably in the neurons of the bladder rather than in the urothelium or detrusor muscle. Furthermore, reactive oxygen species do not interact with this relaxing factor, the exact nature and the physiol...Continue Reading

References

Jul 1, 1991·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·A A ParsonsM Wahl
Jan 17, 1985·The New England Journal of Medicine·J M McCord
Nov 19, 1986·European Journal of Pharmacology·M Ilhan, I Sahin
Jul 1, 1972·British Journal of Pharmacology·J S Gillespie
Sep 9, 1999·European Journal of Pharmacology·I U BurcinM Ilhan
Mar 11, 2000·British Journal of Pharmacology·M H HawthornR Chess-Williams
Apr 2, 2003·Naunyn-Schmiedeberg's Archives of Pharmacology·K InciI Sahin-Erdemli
Jan 6, 2004·Fundamental & Clinical Pharmacology·N Tugba DurluInci Sahin-Erdemli

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Citations

Jul 16, 2008·European Journal of Pharmacology·Turgut Emrah Bozkurt, Inci Sahin-Erdemli
Aug 2, 2006·Autonomic & Autacoid Pharmacology·R M Eglen
May 7, 2009·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Turgut Emrah Bozkurt, Inci Sahin-Erdemli
May 29, 2018·Naunyn-Schmiedeberg's Archives of Pharmacology·Donna SellersMartin C Michel
Apr 4, 2017·Basic & Clinical Pharmacology & Toxicology·Na N GuanKarl Svennersten

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