Evaluation of the RT-LAMP and LAMP methods for detection of Mycobacterium tuberculosis

Journal of Clinical Laboratory Analysis
Dandan WuDianxing Sun

Abstract

The current methods for detecting Mycobacterium tuberculosis (Mtb) are not clinically optimal. Standard culture methods (SCMs) are slow, costly, or unreliable, and loop-mediated isothermal amplification (LAMP) cannot differentiate live Mtb. This study compared reverse transcription (RT)-LAMP, LAMP, and an SCM for detecting Mtb. A first experiment tested the sensitivity and specificity of primers for 9 species of Mycobacterium (H37Rv, M. intracellulare, M. marinum, M. kansasii, M. avium, M. flavescens, M. smegmatis, M. fortuitum, and M. chelonae); and 3 non-Mycobacterium species (Staphylococcus aureus, Pseudomonas aeruginosa, and Klebsiella pneumoniae). A second experiment tested sputum specimens for the presence of Mtb, from 100 patients with tuberculosis (clinical) and 22 from patients without tuberculosis (control), using Roche solid culture (SCM), LAMP, and RT-LAMP. In the clinical samples. The rates of positivity for Mtb of the SCM, LAMP, and RT-LAMP methods were 88%, 92%, and 100%, respectively. The difference in detection rate was significant between RT-LAMP and SCM, but RT-LAMP and LAMP were comparable. In the control group, the detection rates were nil for all three methods. The specificities of the methods were similar...Continue Reading

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Citations

Dec 18, 2018·Nanoscale·Oskar FranchBirgitta Ruth Knudsen
Jan 9, 2019·Analytical and Bioanalytical Chemistry·Xueqin GaoYifu Guan
Mar 12, 2020·Journal of Clinical Laboratory Analysis·Chu-Mao ChenXu-Guang Guo
Oct 25, 2020·The American Journal of Tropical Medicine and Hygiene·Yee Ling LauPik Pin Goh

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