PMID: 29843344Jul 1, 1990Paper

Evaluation of the single tube osmotic fragility test in detection of β-thalassaemia trait

The National Medical Journal of India
A C GorakshakerS Desai

Abstract

NESTROFT (the Naked Eye Single Tube Red Cell Osmotic Fragility Test) is a simpleand inexpensivemethod for detecting the β-thalassaemiatrait in the general population. Its results, however, depend on visual interpretation. We assessed interobserver variability in the recording of results by three individuals on 380 samples of blood collected intravenously. We also tested 745 samples by finger prick using a drop of blood or a uniform (20 μl) volume and found that when a uniform volume was tested the proportion of false positive results was7% (28/380)as compared to 22% (79/365) when a drop was used. We found that although there-was little variation in recording negative results, there was more variation in recording positive or doubtful results.We conclude that when the NESTROFT in field tests is positive or doubtful, then the blood should be further tested in a laboratory for Hb A2 and a uniform volume of blood rather than an arbitrary drop should be used in the test. NESTROFT is suitable for large scale preliminary screening for the β-thalassaemiatrait in India.

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.