Evaluation of the substrate specificity of human mast cell tryptase beta I and demonstration of its importance in bacterial infections of the lung

The Journal of Biological Chemistry
C HuangR L Stevens

Abstract

Human pulmonary mast cells (MCs) express tryptases alpha and beta I, and both granule serine proteases are exocytosed during inflammatory events. Recombinant forms of these tryptases were generated for the first time to evaluate their substrate specificities at the biochemical level and then to address their physiologic roles in pulmonary inflammation. Analysis of a tryptase-specific, phage display peptide library revealed that tryptase beta I prefers to cleave peptides with 1 or more Pro residues flanked by 2 positively charged residues. Although recombinant tryptase beta I was unable to activate cultured cells that express different types of protease-activated receptors, the numbers of neutrophils increased >100-fold when enzymatically active tryptase beta I was instilled into the lungs of mice. In contrast, the numbers of lymphocytes and eosinophils in the airspaces did not change significantly. More important, the tryptase beta I-treated mice exhibited normal airway responsiveness. Neutrophils did not extravasate into the lungs of tryptase alpha-treated mice. Thus, this is the first study to demonstrate that the two nearly identical human MC tryptases are functionally distinct in vivo. When MC-deficient W/W(v) mice were giv...Continue Reading

References

Dec 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·H P McNeilR L Stevens
May 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·P VandersliceG H Caughey
Sep 1, 1990·The Journal of Clinical Investigation·J S MillerL B Schwartz
Apr 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·D S ReynoldsW E Serafin
Oct 1, 1989·The Journal of Clinical Investigation·J S MillerL B Schwartz
Jan 1, 1987·Molecular and Cellular Biology·R B DuBridgeM P Calos
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·R L StevensK F Austen
Sep 15, 1995·Science·D E ShusterM R Ackermann
Sep 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·W S PearD Baltimore
Apr 28, 1995·The Journal of Biological Chemistry·M J WoolkalisL F Brass
Mar 1, 1995·Protein Science : a Publication of the Protein Society·J J Perona, C S Craik
Oct 1, 1994·Mammalian Genome : Official Journal of the International Mammalian Genome Society·M F GurishJ H Nadeau
Mar 17, 1995·The Journal of Biological Chemistry·S NystedtJ Sundelin
May 1, 1994·Scandinavian Journal of Immunology·G NilssonL Hellman
Jan 4, 1994·Proceedings of the National Academy of Sciences of the United States of America·R L StevensK F Austen
Feb 2, 1996·The Journal of Biological Chemistry·J E HuntN Ghildyal
Feb 14, 1997·The Journal of Biological Chemistry·M MolinoL F Brass
Jan 1, 1996·Pathobiology : Journal of Immunopathology, Molecular and Cellular Biology·N W LukacsR K Albert
Sep 18, 1997·The Journal of Clinical Investigation·C U CorveraN W Bunnett
Dec 22, 1998·The Journal of Experimental Medicine·M MaurerS J Galli
Jan 28, 1999·The Journal of Biological Chemistry·M PallaoroG H Caughey
Feb 27, 1999·Current Opinion in Immunology·S J GalliC S Lantz
Mar 17, 1999·The Journal of Clinical Investigation·M L KahnS R Coughlin
Dec 22, 1999·Biochemical and Biophysical Research Communications·M InoueH Kido

❮ Previous
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Citations

Jul 8, 2009·Inflammation·Elena BargagliPaola Rottoli
Aug 5, 2003·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Joshua A Boyce
Feb 6, 2002·Current Opinion in Chemical Biology·Diane J RodiBrian K Kay
Jan 5, 2002·Current Opinion in Pharmacology·F Schmidlin, N W Bunnett
Feb 28, 2002·Trends in Immunology·Frédéric FégerMichel Arock
Apr 17, 2003·Trends in Immunology·Francesca Levi-Schaffer, Adrian M Piliponsky
Oct 2, 2004·Nature Reviews. Immunology·Jean S Marshall
Oct 19, 2001·British Journal of Pharmacology·S J ComptonM D Hollenberg
Aug 10, 2011·Proceedings of the National Academy of Sciences of the United States of America·Wei XingTatiana G Jones
Apr 19, 2012·The Journal of Biological Chemistry·Roberto AdachiRichard L Stevens
Jul 4, 2001·The Journal of Experimental Medicine·M F Gurish, K F Austen
May 24, 2008·Transplantation·Jama JahanyarGeorge P Noon
Apr 16, 2011·Circulation Research·Jie ZhangGuo-Ping Shi
Nov 26, 2009·American Journal of Respiratory Cell and Molecular Biology·Neil N Trivedi, George H Caughey
Feb 18, 2004·The Journal of Clinical Investigation·Jon Mallen-St ClairPaul J Wolters
Nov 20, 2002·Biological Chemistry·David Deperthes
Dec 22, 2010·Proceedings of the National Academy of Sciences of the United States of America·Matthew J HamiltonRichard L Stevens
May 16, 2014·The Journal of Infectious Diseases·Florry E van den BoogaardTom van der Poll
Aug 15, 2014·Respiratory Research·John R GreenlandGeorge H Caughey
May 15, 2007·Immunological Reviews·George H Caughey
Oct 21, 2015·PloS One·Shilpa NimishakaviGeorge H Caughey
Mar 28, 2007·International Immunopharmacology·Runa SurRavi Malaviya
Sep 7, 2005·Biochemical and Biophysical Research Communications·G William Wong, Richard L Stevens
Jul 4, 2006·Protein Expression and Purification·Jing YuanJames M Clark
Apr 3, 2007·Trends in Immunology·Martin Metz, Marcus Maurer
Feb 15, 2005·Archives of Biochemistry and Biophysics·Wilfred W RaymondGeorge H Caughey
Mar 28, 2006·Biochimica Et Biophysica Acta·Yongjie MaLianhua Yin
Nov 18, 2014·Inflammatory Bowel Diseases·Matthew J HamiltonRichard L Stevens
Feb 3, 2004·Journal of Receptor and Signal Transduction Research·Michael Szardenings
Mar 27, 2004·Physiological Reviews·Valeria S Ossovskaya, Nigel W Bunnett
Aug 18, 2010·Laboratory Investigation; a Journal of Technical Methods and Pathology·Tatiana Aparecida PimentelSonia Maria Oliani
Sep 14, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Richard A JohnstonStephanie A Shore
Feb 20, 2009·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Xiao Su, Michael A Matthay
Jan 1, 2010·European Journal of Immunology·Thomas J PalkerWolfgang W Leitner
Jan 16, 2010·ChemMedChem·Norbert Schaschke, Christian P Sommerhoff
Aug 11, 2005·Canadian Journal of Physiology and Pharmacology·Michelle L SeymourWallace K MacNaughton

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