Evaluation of the teratogenicity of fennel essential oil (FEO) on the rat embryo limb buds culture

Toxicology in Vitro : an International Journal Published in Association with BIBRA
S N OstadO Sabzevari

Abstract

The use of FEO as a remedy for control of primary dysmenorrhea increases concern about its potential teratogenicity due to its estrogen-like activity. Limb bud mesenchymal cells, when grown in high-density cultures, can be differentiated into a number of cell types including cartilage and muscle. These cells have been used extensively for in vitro studies of chondrogenesis. Therefore, we used limb bud cells and Alcian blue staining method that is specific for staining cartilage proteoglycan, to determine the teratogenic effect of FEO. Limb bud cells obtained from day 13 rat embryo were cultivated and exposed to various concentrations of FEO for 5 days at 37 degrees C and the number of differentiated foci were counted. Retinoic acid (90 microg/ml) was chosen as positive standard control. The differentiation was also evaluated using limb bud micromass culture using immunocytochemical techniques and BMP-4 antibody. The results showed that FEO at concentration as low as 0.93 mg/ml produced a significant reduction in the number of stained differentiated foci. However, this reduction was due to cell loss, determined by neutral red cell viability assay, rather than to be related to decrease in cell differentiation. These findings sugg...Continue Reading

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Citations

Aug 28, 2014·BioMed Research International·Shamkant B BadgujarAtmaram H Bandivdekar
Jan 1, 2013·Chinese Journal of Integrative Medicine·Roja Rahimi, Mohammad Reza Shams Ardekani
Sep 2, 2015·Journal of Ethnopharmacology·Luigia TrabaceVincenzo Cuomo
May 14, 2019·Journal of Menopausal Medicine·Mohaddese Mahboubi
Mar 7, 2021·International Journal of Molecular Sciences·Noura S Dosoky, William N Setzer

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