Evaluation of the toxic potential of cefotaxime in the third instar larvae of transgenic Drosophila melanogaster

Chemico-biological Interactions
RahulYasir Hasan Siddique

Abstract

The present study was carried out to evaluate the toxic potential of cefotaxime in the third instar larvae of transgenic Drosophila melanogaster (hsp70-lacZ)Bg(9). Cefotaxime at final concentration of 10, 20, 40, 60 and 80 μg/ml was mixed in the diet and the larvae were exposed to the selected doses for 6, 12, 24, 48 h. The hsp70 expression, trypan blue exclusion test, in situ histochemical β-galactosidase activity, lipid peroxidation, total protein content, glutathione (GSH) content, glutathione-S-transferase (GST) activity, protein carbonyl content, caspase 3 and 9 activity, apoptotic index and comet assay were taken as parameters for the study. The larvae exposed to 40, 60 and 80 μg/ml for 12, 24 and 48 h showed a dose and duration dependent significant increase in the activity of β-galactosidase and lipid peroxidation but decrease in the total GSH content as compared to unexposed larvae. The decrease in protein content was observed in the larvae exposed to 40, 60 and 80 μg/ml of cefotaxime for 24 and 48 h. The larvae exposed to 40, 60 and 80 μg/ml of cefotaxime for 24 and 48 h showed a dose and duration dependent increase in the tissue damage, GST, caspase 3 and 9 activity, PC content, apoptosis and the DNA tail length (com...Continue Reading

References

Oct 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·W H HabigW B Jakoby
Sep 1, 1980·The Journal of Antimicrobial Chemotherapy·J ChamberlainJ McEwen
Sep 1, 1980·The Journal of Antimicrobial Chemotherapy·B I DoerrT Sakaguchi
Sep 1, 1980·The Journal of Antimicrobial Chemotherapy·F EsmieuA Le Go
Oct 1, 1981·Antimicrobial Agents and Chemotherapy·D A KafetzisC J Papadatos
Sep 1, 1982·Reviews of Infectious Diseases·R N Jones, C Thornsberry
Sep 1, 1982·Reviews of Infectious Diseases·J D Coombes
Jan 4, 2001·Cell Death and Differentiation·H Y Chan, N M Bonini
Oct 24, 2001·Journal of Biochemical and Molecular Toxicology·D K ChowdhuriD K Saxena
May 3, 2003·Biochimica Et Biophysica Acta·Aamir NazirDebapratim Kar Chowdhuri
Nov 5, 2003·Journal of Biochemical and Molecular Toxicology·Indranil MukhopadhyayD Kar Chowdhuri
May 12, 2005·BMC Biotechnology·Deborah RibbleYiqun G Shellman
Oct 27, 2005·Molecular Interventions·Alicia M Celotto, Michael J Palladino
Jun 13, 2006·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·V PragasamP Varalakshmi
Nov 23, 2006·Antioxidants & Redox Signaling·Stefan W RyterAugustine M K Choi
Mar 18, 2009·Proceedings of the National Academy of Sciences of the United States of America·Henry ChungPhillip J Daborn
May 15, 2009·Free Radical Biology & Medicine·Clare L HawkinsMichael J Davies
Jun 30, 2009·Neurotoxicology and Teratology·Matthew D Rand
Jul 18, 2009·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Yasir Hasan SiddiqueMohammad Afzal
Mar 17, 2012·Dose-response : a Publication of International Hormesis Society·Yasir Hasan SiddiqueMohammad Afzal
Apr 17, 2013·BMC Biology·Akhila Rajan, Norbert Perrimon
Mar 13, 2014·Disease Models & Mechanisms·Edward Owusu-Ansah, Norbert Perrimon

❮ Previous
Next ❯

Citations

Apr 3, 2016·Mutation Research. Reviews in Mutation Research·Maria AugustyniakMarta Dziewięcka
Mar 25, 2016·Basic & Clinical Pharmacology & Toxicology·Hao CaoHelgi B Schiöth
Aug 9, 2017·Environmental Science and Pollution Research International·Maria Paula Mancini CoelhoCarmem Silvia Fontanetti

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis