Evaluation of the Wampole Laboratories ELISA-based assay for Epstein-Barr virus serology

Clinica Chimica Acta; International Journal of Clinical Chemistry
Mark K FungA M Gronowski

Abstract

Epstein-Barr virus (EBV) infection is associated with infectious mononucleosis, Burkitt's lymphoma, and nasopharyngeal carcinoma. Serologic diagnosis of acute EBV infections has been the method of choice, and tests are available as indirect fluorescent antibody (IFA)- and ELISA-based assays. To evaluate the ELISA-based EBV assay from Wampole Laboratories (Cranbury, NJ). One hundred fifty-two consecutive samples received for comprehensive EBV serology were analyzed. A comparison of the Wampole Laboratories' ELISA system with the Gull/Meridian Diagnostics (Cincinnati, OH) IFA, and ELISA assays showed 88% concordance for anti-viral capsid antigen (VCA) IgM (n=177); 79% concordance for anti-VCA IgG (n=177); 87% concordance for anti-NA IgG (n=172); and 48% concordance for anti-EA IgG (n=165). Using the results from all four antibody assays to identify patients with acute infection, the Wampole system had a 67% concordance with the Gull/Meridian (n=164). Differences in the specificity of the anti-EA IgG assays (i.e. reactivity against the D component of early antigen (EA-D) (Wampole) vs. reactivity to EA-D and the R component of early antigen (EA-R) (Gull/Meridian)) may have lead to poor concordance (48%) for this particular assay. B...Continue Reading

References

Oct 6, 2000·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·G A Storch
Dec 15, 2000·Archives of Family Medicine·K E Burroughs

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Citations

Sep 26, 2009·Clinical and Experimental Medicine·Omer CoskunCan Polat Eyigun
Aug 1, 2012·Gastroenterology Nursing : the Official Journal of the Society of Gastroenterology Nurses and Associates·Isilay NadirCansel Turkay
Nov 6, 2004·Journal of Clinical Microbiology·J S KluttsA M Gronowski
Jul 18, 2008·Clinical and Vaccine Immunology : CVI·M J BinnickerE M Beito

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