Evaluation of three automated human immunoturbidimetric assays for the detection of C-reactive protein in dogs

Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
Stefanie KlennerAndreas Moritz

Abstract

C-reactive protein (CRP) is a major, acute-phase protein in dogs; however, there is a need for automated assays to ensure in-time patient monitoring. Three automated immunoturbidimetric assays (Randox, Thermo, and Wako) developed for human beings were evaluated for their ability to detect canine CRP, including method validation, evaluation of diagnostic use, and establishment of exploratory reference intervals. Sera from 36 healthy dogs and 82 diseased dogs were included for method comparison with the enzyme-linked immunosorbent assay (ELISA; Tridelta) serving as the reference method. A nonparametric estimate of the 1-sided 95% reference interval was established (n = 36). Precision study revealed good intra-assay coefficients of variation (CVs) of 1-10%, 0-9%, and 2-13% for the Randox, Thermo, and Wako assays, respectively. Interassay CVs were 18%, 24%, and 19% respectively. Because of a low linear range, the Thermo test was considered unsuitable for use with canine specimens. No significant differences were present between the results obtained with the Randox and Wako assays with CRP concentrations less than 15 mg/l; however, median CRP results differed significantly between the Thermo test and the ELISA (P = 0.03). Bland-Altm...Continue Reading

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Citations

Jan 5, 2011·Veterinary Clinical Pathology·Mads Kjelgaard-Hansen
Feb 4, 2016·Veterinary Immunology and Immunopathology·Iulia KarlssonSara Wernersson
Jul 19, 2011·Research in Veterinary Science·Natali BauerAndreas Moritz
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Jan 13, 2017·Journal of Zoo and Wildlife Medicine : Official Publication of the American Association of Zoo Veterinarians·Michael W HyattCarolyn Cray
Jul 25, 2014·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Ramiro IsazaCarolyn Cray
Dec 18, 2012·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Carolyn CrayGregory D Bossart
Jan 30, 2018·Veterinary Clinical Pathology·Matilde PiñeiroFrancesca Canalias
Sep 18, 2018·Journal of Veterinary Internal Medicine·Romy M Heilmann, Jörg M Steiner
May 13, 2015·Veterinary Clinical Pathology·Anne-Katherine JasenskyBarbara Kohn
Sep 24, 2020·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Michele BerlandaHelen Poser
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Jul 28, 2017·Veterinary Clinical Pathology·Saverio Paltrinieri, Elisa Talon
Mar 13, 2021·Journal of Veterinary Internal Medicine·Aude Morgane CanonneCecile Clercx
Nov 13, 2021·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Julia KiemleMichael Roecken

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Methods Mentioned

BETA
enzyme-linked
ELISAs
ELISA
biopsy
glycosylation

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