PMID: 8978097Dec 15, 1996Paper

Evaluation of three individual glucosyltransferases produced by Streptococcus mutans using monoclonal antibodies

FEMS Microbiology Letters
Y TomitaK Fukushima

Abstract

We previously established murine hybridomas producing a monoclonal antibody monospecific against three glucosyltransferases (I, SI and S) of Streptococcus mutans which contribute to dental caries formation. Here, we developed a new immunochemical technique (cross-dot system) with which individual levels of glucosyltransferases expressed by S. mutans can be evaluated. We also examined glucosyltransferase production and in vitro artificial plaque formation by a reference strain and several clinical isolates of S. mutans. The findings indicate that the levels of glucosyltransferases produced greatly vary with the cells and the culture medium, and that the cells producing high levels of both glucosyltransferase-SI and glucosyltransferase-I enzymes may possess high in vitro artificial plaque forming ability. We suggest that the cross-dot system will be useful for estimating the cariogenic potential of S. mutans isolates.

References

Jul 1, 1992·Infection and Immunity·K FukushimaH K Kuramitsu
Dec 15, 1992·Biochemical and Biophysical Research Communications·C KatoH K Kuramitsu

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Citations

Mar 17, 2004·FEMS Microbiology Letters·Ramona RozenGilad Bachrach
Aug 4, 2019·BMC Complementary and Alternative Medicine·Kiranmai MandavaNamratha Sunkara
Jul 29, 2011·Clinical and Vaccine Immunology : CVI·Tomonori HoshinoTaku Fujiwara

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