Evaluation of three molecular carbapenemase tests: Eazyplex SuperBug complete B, Novodiag CarbaR+, and Amplidiag CarbaR+MCR.

Journal of Microbiological Methods
Tanja HolmaJohanna Haiko

Abstract

Carbapenemase-producing Gram-negative bacilli, i.e., Enterobacterales, Pseudomonas aeruginosa, and Acinetobacter, are of increased concern for the public health around the world. There is urgent need for rapid and accurate tests in order to provide correct treatment and to prevent bacterial spread in healthcare settings. The aim of this study was to evaluate three commercial multiplex carbapenemase tests with CE-IVD marking: Eazyplex SuperBug complete B (AmplexDiagnostics), Novodiag CarbaR+ (Mobidiag), and Amplidiag CarbaR+MCR (Mobidiag). All these tests recognize KPC, NDM, OXA-48/181 group, VIM, OXA-23 group, and OXA-24/40 group, and Novodiag CarbaR+ and Amplidiag CarbaR+MCR additionally recognize IMP, OXA-51 group (with promoter located within ISAbaI), OXA-58 group, and MCR, and Amplidiag CarbaR+MCR further recognizes GES (carbapenemase-type only). The sensitivities and specificities of these tests with bacterial isolates were 100%. The sensitivity directly from clinical samples was 100%, but the specificity was lower, which is simply explained by the higher sensitivity of the molecular methods compared with culture method. Overall, these CE-IVD marked tests provide a good alternative in the detection of carbapenemase-produci...Continue Reading

References

Aug 8, 2015·Journal of Applied Microbiology·J Osei SekyereS Y Essack
Nov 22, 2016·The Lancet Infectious Diseases·Hajo GrundmannUNKNOWN European Survey of Carbapenemase-Producing Enterobacteriaceae (EuSCAPE) Working Group
Jan 13, 2017·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·L Lapointe-ShawA McGeer
May 2, 2017·Clinics in Laboratory Medicine·Alina Iovleva, Yohei Doi
Jan 7, 2018·Journal of Clinical Microbiology·Saoussen OueslatiThierry Naas
Mar 23, 2018·Nature Communications·Ruobing WangFrancois Balloux
Aug 31, 2018·Journal of Clinical Microbiology·Pranita D Tamma, Patricia J Simner
May 21, 2020·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Delphine GirlichThierry Naas

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