Evaluation of three transporter ligands as quantitative markers of serotonin innervation density in rat brain

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L DescarriesR Tanguay

Abstract

Direct counting of axon terminals (varicosities) labeled by uptake/storage of a tritiated monoamine provides a means to test radioligands of the corresponding membrane transporter as quantitative markers of regional monoamine innervation density in brain tissue. In autoradiographs from alternate rat brain slices, counts of [3H]5-HT-labeled axon terminals were matched with densitometric measurements of the specific binding of tritiated cyanoimipramine (CYI), citalopram (CITAL), and 6-nitroquipazine (6-NTQ), under conditions of hypo-, normo-, or hyper-5-HT innervation of the neostriatum. A total of 267 pairs of data were subjected to a multilevel analysis (iterative generalized least square procedure). With all three ligands, there was a linear relationship between the density of 5-HT innervation and the density of specific binding and no change in the slope of the regression lines as a function of 5-HT innervation density. Thus, none of these ligands gave any sign of down- or up-regulation of the 5-HT transporter consequent to 5-HT hypo- or hyper-innervation. The regression lines for CYI and CITAL were not significantly different from one another and crossed the ordinate near zero, whereas the regression line for 6-NTQ was less ...Continue Reading

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Citations

Jul 2, 2003·Neurotoxicity Research·Richard M KostrzewaRyszard Brus
Nov 13, 2007·Journal of Neural Transmission·R M KostrzewaR Brus
May 29, 1998·Brain Research. Developmental Brain Research·S Mansour-RobaeyL Descarries
Sep 9, 1999·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Z SzaboR F Dannals
Oct 1, 2010·Annals of Neurology·Daniella RylanderM Angela Cenci
Mar 30, 2010·The European Journal of Neuroscience·Martin ParentLaurent Descarries

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