Evaluation of tissue-specific promoters in carcinomas of the cervix uteri

The Journal of Gene Medicine
Daniel T ReinDavid T Curiel

Abstract

Gene therapy is a novel approach for treatment of patients with advanced, recurrent, or metastatic cervical cancer. One effective way to direct transgene expression to specific tissues or tumors is the use of tissue-specific-promoters (TSPs). In the context of adenovirus (Ad)-mediated cancer gene therapy it is rational to choose a TSP which is highly expressed in the tumor but has potentially low activity in non-tumor cells, especially the liver. In this study, we have investigated several promoters which fulfill these criteria. Candidate cervical cancer specific TSPs include promoters of the genes for secretory leukoprotease inhibitor (SLPI), cyclooxygenase-2 (COX-2), Midkine (MK), vascular endothelial growth factor receptor type 1 (flt-1), vascular endothelial growth factor (VEGF), Survivin and the receptor for chemokine SDS-1 (CXCR4). To evaluate the specific gene expression of the different promoters in the context of cervical cancer, we constructed a panel of E1-deleted Ads that express luciferase under the control of the promoters of interest. We investigated various established cervical cancer cell lines, as well as purified primary cancer cells and normal control cells from the cervix uteri. In all cell lines tested, pr...Continue Reading

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Citations

Sep 4, 2008·Molecular Therapy : the Journal of the American Society of Gene Therapy·Sunetra RaySanjiv S Gambhir
Jul 7, 2012·Journal of Cellular Physiology·Nicolás O AmianoH Eduardo Chuluyan
Feb 4, 2006·The Lancet Oncology·Dominique BouchardGuy M Tremblay
Mar 16, 2007·Molecular Therapy : the Journal of the American Society of Gene Therapy·Joseph D MocanuFei-Fei Liu
Sep 1, 2006·American Journal of Physiology. Cell Physiology·Ilona KryczekWeiping Zou
Jul 26, 2016·Oncology Letters·Mikhail V ShepelevIgor V Korobko

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