Evaluation of total plasma nitric oxide concentrations in broilers infused intravenously with sodium nitrite, lipopolysaccharide, aminoguanidine, and sodium nitroprusside

Poultry Science
M E Chapman, R F Wideman

Abstract

Nitric oxide (NO) is a potent vasodilator that is synthesized by constitutive and inducible isoforms of the enzyme NO synthase (eNOS and iNOS, respectively). The half-life of NO averages only 3 to 4 s in biological fluids, where it is rapidly converted to the stable oxidation products nitrite (NO2-) and nitrate (NO3-). Our objectives were to use 2 commercial kits to measure total plasma NO, as NO2- + NO3-, and to assess plasma NO values during experimental protocols designed to influence NO accumulation in the plasma. One kit employed copper-coated cadmium as a catalyst for reducing NO3- to NO2-; the second kit employed the enzyme NO3- reductase for the same purpose. Both then employed Griess reagent for the colorimetric determination of NO2- as a measure of total plasma NO. Broilers in Experiment 1 were infused i.v. with solutions containing increasing concentrations of sodium NO2-. Broilers in Experiment 2 were injected with 1 mg of lipopolysaccharide (LPS), which is known to stimulate iNOS activity. Both commercial kits successfully detected increases in total plasma NO attributable to ongoing i.v. NO2- infusion or to increased iNOS expression at 5 h after the LPS injection. In Experiment 3, we compared the total plasma NO r...Continue Reading

Citations

May 30, 2009·Veterinary Immunology and Immunopathology·O T BowenG F Erf
Jan 11, 2011·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Mare LõhmusMats Björklund
Jan 5, 2012·Biochemical and Biophysical Research Communications·Mika YanagitaiTakumi Satoh
Oct 17, 2013·Journal of Dental Research·T SugiyamaK Iwata
Feb 20, 2020·International Journal of Molecular Sciences·Jun LeeKoichi Iwata
Mar 18, 2016·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Valter DantonioKênia C Bícego
Mar 2, 2011·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Koji FujitaAtsushi Nakajima
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