PMID: 9657285Jul 10, 1998Paper

Evaluation of toxicity indicators in rat primary astrocytes, C6 glioma and human 1321N1 astrocytoma cells: can gliotoxicity be distinguished from cytotoxicity?

Archives of Toxicology
C Mead, V W Pentreath

Abstract

A comparison was made of rat primary astrocytes, C6 glioma cells pre-treated with dibutyryl cyclic AMP, and the human astrocyte 132N1 cell line using a range of 40 compounds and the neutral red (NR) assay. The 40 chemicals included substances known to be toxic to astrocytes or neurons, to be generally cytotoxic or not thought to be toxic to nervous tissue. For those compounds which were toxic, changes in glial fibrillary acidic protein (GFAP) levels were measured in the primary and C6 cultures, and changes in vimentin and S-100 measured in the C6 cells. The number of compounds with EC50 values < 2000 microg/ml for the NR assay for the different cell cultures were as follows: primary astrocytes, 19; C6 cells, 15; and 1321N1 cells, 11. The log of the EC50 values for the NR assay for the test compounds between the three cell types was not significantly different at the 5% level by paired Student's t-test. For the toxic substances the correlation coefficients of the EC50 values between primary cells and the C6 or 1321N1 cells were r > 0.5, and between the C6 and 1321N1 cells r > 0.9. For GFAP there was a similar degree of correlation in EC50 values between the different cell types. The GFAP, vimentin and S-100 levels showed similar...Continue Reading

Citations

Dec 21, 2011·Apoptosis : an International Journal on Programmed Cell Death·Yu Mi HaKi Churl Chang
Jan 28, 2009·Cellular and Molecular Neurobiology·Larissa PuschGustav F Jirikowski
Sep 1, 2000·Chemico-biological Interactions·N D Slamon, V W Pentreath
Aug 6, 2002·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·E-M FröjdöB Isomaa
May 7, 2009·Journal of Visualized Experiments : JoVE·Janet L AnderlAndrew J Ball
Jun 18, 2003·Critical Reviews in Toxicology·Edward J Calabrese, Linda A Baldwin
Nov 1, 2008·Environmental Toxicology and Pharmacology·Lindsay J Holden, Michael D Coleman
Jan 22, 2014·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Jiann-Gwu LeeChin-Cheng Chou
Jan 1, 2005·Nonlinearity in Biology, Toxicology, Medicine·N Debbie SlamonVic W Pentreath
Jan 1, 2004·Nonlinearity in Biology, Toxicology, Medicine·V W Pentreath, C Mead
Jun 23, 2010·Environmental Management·Daniel LavenNora Mitchell

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