Evaluation of TPGS-modified thermo-sensitive Pluronic PF127 hydrogel as a potential carrier to reverse the resistance of P-gp-overexpressing SMMC-7721 cell lines

Colloids and Surfaces. B, Biointerfaces
Lei GaoGuiyang Liu

Abstract

In the present studies locally injectable docetaxel nanocrystals loaded d-alpha tocopheryl polyethylene glycol 1000 succinate-modified Pluronic F127 (DOC-NCs-TPGS-PF127) thermo-sensitive hydrogels were prepared to reverse drug resistance of P-glycoprotein (P-gp)-overexpressing human liver cancer SMMC-7721 tumors. Firstly, DOC nanosuspensions with mean particle size of 196nm were prepared and dispersed into series of mixed solutions containing PF127 and TPGS of different ratios to obtain DOC-NCs-TPGS-PF127 hydrogels. DOC NCs, exhibiting a uniform distribution and very good physical stability during three sol-gel cycles in the hydrogel network, did not influence the gelation temperature. Swelling-dependent release pattern was found for DOC NCs from hydrogels and release profiles could be well fitted by the Peppas equation. MTT test showed that hydrogels containing 0% or 0.1% TPGS had no cytotoxicity against L929 fibroblasts. Both DOC solution and DOC-NCs-TPGS-PF127 hydrogels exhibited obvious cytotoxicity against sensitive SMMC-7721 cells. When resistant SMMC7721 cells were treated, DOC-NCs-TPGS-PF127 hydrogels showed significantly higher cytotoxicity compared with DOC solution and hydrogels containing no TPGS (DOC-NCs-PF127), wi...Continue Reading

References

Feb 21, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Takeharu OnoKyogo Itoh
Jan 9, 2007·Journal of Controlled Release : Official Journal of the Controlled Release Society·Yu LiuQiang Zhang
Jul 27, 2010·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Armelle Aka-Any-GrahGilles Ponchel
Mar 24, 2012·Colloids and Surfaces. B, Biointerfaces·Sachin GahoiFarhan J Ahmad
Mar 29, 2014·Colloids and Surfaces. B, Biointerfaces·Lei GaoGuifang Dou
May 24, 2014·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Aditya GanjuMeena Jaggi
Oct 4, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·Zhigui SuCan Zhang
Feb 13, 2015·Biomacromolecules·Jennifer M KnipeNicholas A Peppas

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Citations

Jul 18, 2016·Drug Discovery Today·Mohammad NorouziDonald W Miller
Mar 25, 2017·Journal of Pharmaceutical Sciences·Hanif HaidariSanjay Garg
Sep 25, 2019·Pharmaceutics·Giuseppe CirilloFrancesca Iemma
May 15, 2018·Advanced Materials·Sepehr TalebianGordon G Wallace
Feb 23, 2021·Toxicon : Official Journal of the International Society on Toxinology·Bunyamin TrasKamil Uney
Aug 1, 2021·International Journal of Pharmaceutics·Diana RafaelIbane Abasolo

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