Evaluation of transfection efficiency in skeletal muscle using nano/microbubbles and ultrasound

Ultrasound in Medicine & Biology
T KodamaM Fukumoto

Abstract

Recent studies have revealed that ultrasound contrast agents with low-intensity ultrasound, namely, sonoporation, can noninvasively deliver therapeutic molecules into target sites. However, the efficiency of molecular delivery is relatively low and the methodology requires optimization. Here, we investigated three types of nano/microbubbles (NMBs)-human albumin shell bubbles, lipid bubbles and acoustic liposomes-to evaluate the efficiency of gene expression in skeletal muscle as a function of their physicochemical properties and the number of bubbles in solution. We found that acoustic liposomes showed the highest transfection and gene expression efficiency among the three types of NMBs under ultrasound-optimized conditions. Liposome transfection efficiency increased with bubble volume concentration; however, neither bubble volume concentration nor their physicochemical properties were related to the tissue damage detected in the skeletal muscle, which was primarily caused by needle injection.

References

Jan 1, 1997·Ultrasound in Medicine & Biology·S BaoD L Miller
Oct 29, 2000·Ultrasound in Medicine & Biology·M WardJ F Chiu
Apr 5, 2001·Trends in Immunology·G J Prud'hommeA N Theofilopoulos
Nov 26, 2002·Cancer Letters·Tetsuya KodamaMichael R Hamblin
Feb 1, 2003·The Journal of the Acoustical Society of America·Wen-Shiang ChenLawrence A Crum
May 14, 2004·Gene Therapy·R J CritchleyG Vassaux
Jun 3, 2004·Nature Reviews. Drug Discovery·Jonathan R Lindner
Oct 26, 2005·Protein Engineering, Design & Selection : PEDS·Elyse ShapiroFrançois Baneyx
Jan 21, 2006·IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control·Mark A BordenKatherine W Ferrara
Apr 6, 2006·Chemical & Pharmaceutical Bulletin·Kaname HashizakiNaotake Ogawa
Jun 21, 2006·Ultrasound in Medicine & Biology·Tetsuya KodamaMartin J K Blomley
Jun 21, 2006·Ultrasound in Medicine & Biology·Robyn K SchlicherMark R Prausnitz
Jul 11, 2006·Ultrasound in Medicine & Biology·Daniel M HallowMark R Prausnitz
Feb 3, 2007·Journal of Controlled Release : Official Journal of the Controlled Release Society·Chien-Wen ChangSung Wan Kim
Apr 7, 2007·Ultrasound in Medicine & Biology·Denise A B SmithChristy K Holland
Jun 15, 2007·Biochemical and Biophysical Research Communications·Manabu Kinoshita, Kullervo Hynynen
Dec 22, 2007·Ultrasound in Medicine & Biology·Atsuko AoiTetsuya Kodama
Jan 15, 2008·Biophysical Journal·Hai-Lei ZhuNoriyoshi Teramoto
May 20, 2008·Advanced Drug Delivery Reviews·Sophie Hernot, Alexander L Klibanov
Nov 21, 2008·Cancer Science·Yukiko WatanabeTetsuya Kodama
Dec 5, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Kanwal Tanwir, Valeria Tsoukanova

❮ Previous
Next ❯

Citations

Dec 3, 2014·Colloids and Surfaces. B, Biointerfaces·Ghaleb A HusseiniAna M Martins
Jun 5, 2013·Molecular Therapy : the Journal of the American Society of Gene Therapy·Misty L NobleCarol H Miao
Oct 11, 2011·Journal of Drug Targeting·Anna G SoraceKenneth Hoyt
Jul 1, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·Pedro Gomes SanchesHolger Grüll
May 25, 2012·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·Joshua OwenEleanor Stride
Feb 1, 2015·Ultrasound in Medicine & Biology·Shigeki KatoTetsuya Kodama
Sep 5, 2012·Pharmaceutical Research·Nicolas Sax, Tetsuya Kodama
Feb 5, 2021·International Journal of Pharmaceutics·Shigeki KatoTetsuya Kodama

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.