Evaluation of Trapped Ion Mobility Spectrometry Source Conditions Using Benzylammonium Thermometer Ions

Journal of the American Society for Mass Spectrometry
Cameron N NaylorBrian H Clowers

Abstract

A key aspect of reduced pressure ion mobility spectrometry (IMS) experiments is to identify experimental conditions that minimize the role of collisional energy transfer that allows for assessing effective ion-neutral collision cross sections of metabolites, peptides, and proteins in "native-like" or compact states. Across two separate experimental campaigns using a prototype trapped ion mobility spectrometer (TIMS) coupled to a time-of-flight mass spectrometer, we present independent findings that support the results recently published by Morsa et al. using a different set of thermometer ions (Morsa et al. Anal. Chem. 2020, 92 (6), 4573-4582). First, using five para-substituted benzylammonium ions, we conducted survival yield experiments to assess ion internal energy across different experimental settings. Results from the present set of experiments illustrate that greater ion heating occurs at lower pressures and higher voltage settings applied to the TIMS. At the "softest" settings where the benzylammonium thermometer ions have an effective average energy of 1.73 eV, we observe the majority of bradykinin in the compact state. Under more extreme operating conditions where the energy of the benzylammonium ions varies from 1.83...Continue Reading

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Citations

Feb 25, 2021·Analytical Chemistry·Aurélien Le FèvreFabien Chirot
Jul 15, 2021·Analytical Chemistry·Nicholas B Borotto, Katherine A Graham
Aug 21, 2021·Molecular & Cellular Proteomics : MCP·Florian MeierMatthias Mann
Dec 7, 2021·Journal of the American Society for Mass Spectrometry·Nicholas B BorottoKatherine A Graham

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