Evaluation of trehalose-mannitol broth for differentiation of Staphylococcus epidermidis from other coagulase-negative staphylococcal species.

Journal of Clinical Microbiology
C C Knapp, J A Washington

Abstract

Trehalose-mannitol agar and various broth formulations thereof were evaluated for the differentiation of Staphylococcus epidermidis from other common coagulase-negative staphylococcal species isolated from human blood cultures. While this differentiation was accurately made on trehalose-mannitol agar in 18 h, modification of the medium to a broth formulation containing 2% each trehalose and mannitol in 0.2 ml of purple broth base provided greater than 95% accuracy when a heavy inoculum was incubated on a rotary shaker for at least 4 h.

References

Jan 1, 1975·Journal of Clinical Microbiology·W E Kloos, K H Schleifer
Apr 1, 1987·American Journal of Clinical Pathology·G L WoodsC C Knapp

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Citations

Mar 12, 2005·Memórias do Instituto Oswaldo Cruz·Maria de Lourdes R S CunhaLiciana V A Silveira

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