Evaluation of two molecular techniques for rapid detection of the main dermatophytic agents of tinea capitis

The British Journal of Dermatology
S DengW Liao

Abstract

Tinea capitis is very common in Western China, with the most widespread aetiological agent being Trichophyton violaceum, while Microsporum canis is prevalent in the remainder of China. Conventional diagnostics and internal transcribed spacer (ITS) sequencing analyses have proven relatively limited due to the close phylogenetic relationship of anthropophilic dermatophytes. Therefore, alternative molecular tools with sufficient specificity, reproducibility and sensitivity are necessary. To evaluate two molecular techniques [multiplex ligation-dependent probe amplification (MLPA) and rolling circle amplification (RCA)] for rapid detection of the aetiological agents of tinea capitis, T. violaceum and M. canis. Probes of RCA and MLPA were designed with target sequences in the rDNA ITS gene region. Strains tested consist of 31 T. violaceum, 22 M. canis and 24 reference strains of species that are taxonomically close to the target species. The specificity and reproducibility of RCA and MLPA in detection of T. violaceum and M. canis were both 100% in both species. Sensitivity testing showed that RCA was positive at concentrations down to 1·68 × 10(6) copies of DNA in the TvioRCA probe, and 2·7 × 10(8) copies of DNA in McRCA. MLPA yield...Continue Reading

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Citations

Dec 29, 2015·The British Journal of Dermatology·R J Hay
Mar 24, 2016·The British Journal of Dermatology·R J Hay
Sep 8, 2016·Mycopathologia·R J Hay
Sep 26, 2020·Journal of the European Academy of Dermatology and Venereology : JEADV·C Rodríguez-CerdeiraD M Saunte

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