Evaluation of two novel barcodes for species recognition of opportunistic pathogens in Fusarium

Fungal Biology
Abdullah M S Al-HatmiC André Levesque

Abstract

The genus Fusarium includes more than 200 species of which 73 have been isolated from human infections. Fusarium species are opportunistic human pathogens with variable aetiology. Species determination is best made with the combined phylogeny of protein-coding genes such as elongation factor (TEF1), RNA polymerase (RPB2) and the partial β-tubulin (BT2) gene. The internal transcribed spacers 1, 2 and 5.8S rRNA gene (ITS) have also been used, however, ITS cannot discriminate several closely related species and has nonorthologous copies in Fusarium. Currently, morphological approaches and tree-building methods are in use to define species and to discover hitherto undescribed species. Aftter a species is defined, DNA barcoding approaches can be used to identify species by the presence or absence of discrete nucleotide characters. We demonstrate the potential of two recently discovered DNA barcode loci, topoisomerase I (TOP1) and phosphoglycerate kinase (PGK), in combination with other routinely used markers such as TEF1, in an analysis of 144 Fusarium strains belonging to 52 species. Our barcoding study using TOP1 and PKG provided concordance of molecular data with TEF1. The currently accepted Fusarium species sampled were well sup...Continue Reading

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Citations

Jan 20, 2016·Fungal Biology·Kittipan SamerpitakG Sybren de Hoog
Aug 3, 2016·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Rebecca YahrBryn T M Dentinger
Apr 8, 2016·PLoS Pathogens·Abdullah M S Al-HatmiG Sybren de Hoog
Feb 16, 2017·Journal of Natural Products·Huzefa A RajaNicholas H Oberlies
Jun 12, 2019·FEMS Microbiology Ecology·José F Cobo-DíazAdeline Picot
Oct 31, 2018·Scientific Reports·Danny HaelewatersDonald H Pfister
Mar 18, 2017·Antonie van Leeuwenhoek·Tarek A A MoussaAbdullah M S Al-Hatmi
Jan 1, 2018·European Journal of Plant Pathology·Moslem PapizadehHossein Ramezani
Aug 16, 2019·Fungal Genetics and Biology : FG & B·Liang ZhaoAbdullah M S Al-Hatmi

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