Evaluation of two recombinant Leishmania proteins identified by an immunoproteomic approach as tools for the serodiagnosis of canine visceral and human tegumentary leishmaniasis

Veterinary Parasitology
Eduardo A F CoelhoCarlos Alberto Pereira Tavares

Abstract

Serological diagnostic tests for canine and human leishmaniasis present problems related with their sensitivity and/or specificity. Recently, an immunoproteomic approach performed with Leishmania infantum proteins identified new parasite antigens. In the present study, the diagnostic properties of two of these proteins, cytochrome c oxidase and IgE-dependent histamine-releasing factor, were evaluated for the serodiagnosis of canine visceral (CVL) and human tegumentary (HTL) leishmaniasis. For the CVL diagnosis, sera samples from non-infected dogs living in an endemic or non-endemic area of leishmaniasis, sera from asymptomatic or symptomatic visceral leishmaniasis (VL) dogs, from Leish-Tec(®)-vaccinated dogs, and sera from animals experimentally infected by Trypanosoma cruzi or Ehrlichia canis were used. For the HTL diagnosis, sera from non-infected subjects living in an endemic area of leishmaniasis, sera from active cutaneous or mucosal leishmaniasis patients, as well as those from T. cruzi-infected patients were employed. ELISA assays using the recombinant proteins showed both sensitivity and specificity values of 100% for the serodiagnosis of both forms of disease, with high positive and negative predictive values, showing ...Continue Reading

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Citations

Feb 28, 2019·International Microbiology : the Official Journal of the Spanish Society for Microbiology·Pedro J AlcoleaVicente Larraga
Aug 23, 2019·Revista Do Instituto De Medicina Tropical De São Paulo·Andernice Dos Santos ZanettiOmar Ariel Espinosa
Oct 20, 2020·Parasitology·Joyce Carvalho PereiraVanete Thomaz Soccol
Oct 28, 2020·Microorganisms·Juliana Quero ReimãoAdriano Cappellazzo Coelho

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