Evaluation of urothelial stretch-induced cyclooxygenase-2 expression in novel human cell culture and porcine in vivo ureteral obstruction models

The Journal of Pharmacology and Experimental Therapeutics
Travis J JerdeStephen Y Nakada

Abstract

Obstruction and stretch induce cyclooxygenase (COX)-2 expression and prostanoid synthesis in urinary tissues, causing pain, inflammation, hypercontractility, and cell proliferation. Our objective was to characterize acute COX-2 induction during in vivo ureteral obstruction, establish a cell culture model of urothelial stretch-induced COX-2 expression, and evaluate whether mechanotransduction could alter transcriptional and post-transcriptional regulation of COX-2. We performed laparoscopic unilateral ureteral ligation in pigs and allowed progression for 1, 2, 6, 24, or 48 h. We evaluated COX-2 expression with reverse transcriptase (RT)-polymerase chain reaction (PCR) and immunoblotting. We cultured primary human urothelial cells on stretch plates, applied stretch for up to 48 h, and measured COX-2 expression by RT-PCR and immunoblotting, transcription with run-on assays, and mRNA stability with actinomycin mRNA decay assays. In vivo ureteral obstruction induced COX-2 expression 4-fold within 6 h, maintaining induction for 24 h. In cell culture, stretch induced COX-2 steady-state mRNA and protein within the first 3 h of stretch, maintaining this induction for over 6 h. Three hours of stretch doubled COX-2 transcription relative ...Continue Reading

References

Jan 1, 1991·International Urology and Nephrology·I BaşarD Remzi
Jan 1, 1988·British Journal of Urology·R S ColeK E Shuttleworth
Dec 1, 1995·The Journal of Urology·J Y ClarkS A Optenberg
Jul 1, 1994·The Annals of Pharmacotherapy·R Oren, M Ligumsky
Feb 20, 1993·Biochimica Et Biophysica Acta·K Mangasarian, W S Mellon
Mar 1, 1996·British Journal of Pharmacology·K SeibertD Salvemini
Feb 1, 1997·Annals of Internal Medicine·H I FeldmanB L Strom
May 23, 1998·The Journal of Biological Chemistry·A G SpencerW L Smith
Aug 7, 1998·Urological Research·M AliO Thulesius
Aug 31, 2001·JAMA : the Journal of the American Medical Association·D MukherjeeE J Topol
Mar 7, 2002·American Journal of Physiology. Cell Physiology·Randy C MifflinDon W Powell
Sep 17, 2002·Arteriosclerosis, Thrombosis, and Vascular Biology·Hiroyasu InoueToshiyuki Sasaguri
Jul 30, 2003·The Journal of Experimental Medicine·Dan A DixonStephen M Prescott
Dec 3, 2003·American Journal of Physiology. Cell Physiology·Priya PrahaladKarl S Matlin
Feb 11, 2004·Proceedings of the National Academy of Sciences of the United States of America·Kristine PhillipsPaul Anderson
Feb 18, 2004·Current Pharmaceutical Design·Dan A Dixon

❮ Previous
Next ❯

Citations

Jul 31, 2013·Biochemical Pharmacology·Gaurav Jain, Edgar A Jaimes
May 20, 2008·Journal of Endourology·Gaurav BandiStephen Y Nakada
Sep 25, 2012·Journal of Endourology·Kwadwo Owusu-OforiStephen Y Nakada
Jul 9, 2010·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Inge CarlsenRikke Nørregaard
Jun 18, 2011·The Prostate·Bayli J BoehmWade Bushman

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.