Evaluation of UVA Cytotoxicity for Human Endothelium in an Ex Vivo Corneal Cross-linking Experimental Setting

Journal of Refractive Surgery
Pepijn MoorenCarina Koppen

Abstract

To evaluate endothelial cytotoxicity after exposure of human corneas to ultraviolet-A (UVA) (λ = 365 nm; 5.4 J/cm(2)) in an experimental ex vivo corneal cross-linking setting. Sixteen pairs of human donor corneas were cut into two pieces. One piece of each cornea was treated with 0.025% riboflavin solution prior to UVA irradiation (5 minutes; 18 mW/cm(2)), whereas the other piece was not irradiated but treated with riboflavin (right eye) or preservation medium (left eye). By irradiating from the endothelial side, the UVA dosage applied to endothelial cells exceeded at least eight times the cytotoxic threshold established in animal models (0.65 J/cm(2)). Endothelial cell counts were performed by two independent investigators after storage (4 to 5 days at 31 °C) and staining (trypan blue, alizarin red). Normality (Q-Q plot; Shapiro-Wilk test) and equivalence (mixed-effects modeling with a 10% equivalence threshold) of the endothelial cell counts of the different groups were evaluated. Equivalence of mean endothelial cell density between both groups was observed: 2,237 ± 208 cells/mm(2) in UVA-irradiated pieces and 2,290 ± 281 cells/mm(2) in control pieces (mean difference of 53 ± 240 cells/mm(2) between both groups). Despite dire...Continue Reading

References

Apr 30, 2003·American Journal of Ophthalmology·Gregor WollensakTheo Seiler
Jul 11, 2003·Progress in Retinal and Eye Research·Nancy C Joyce
Oct 3, 2003·Journal of Cataract and Refractive Surgery·Gregor WollensakTheo Seiler
Dec 23, 2003·Ophthalmic Research·Gregor WollensakRichard Funk
Oct 27, 2004·Investigative Ophthalmology & Visual Science·Pervinder SagooAndrew J T George
Apr 26, 2007·Cornea·Eberhard SpoerlTheo Seiler
Jan 29, 2010·Eye & Contact Lens·Carina KoppenMarie-José Tassignon
Feb 2, 2010·Journal of Cataract and Refractive Surgery·Gregor WollensakSaadettin Sel
Jul 6, 2011·American Journal of Ophthalmology·Juha M Holopainen, Kari Krootila
Aug 25, 2011·American Journal of Ophthalmology·George D KymionisMichael A Grentzelos
Apr 26, 2012·Cornea·Sunju ParkRoy S Chuck
Aug 8, 2012·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Clemens LangeThomas Reinhard
Sep 11, 2012·American Journal of Ophthalmology·Ashok SharmaVerinder Singh Nirankari
Mar 29, 2014·Investigative Ophthalmology & Visual Science·Arthur HammerFarhad Hafezi
May 27, 2014·Cornea·Mohamed-Sameh H El-AghaHamdy M Essam

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Citations

Apr 7, 2018·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Sandeepani K SubasingheGeorge J Dias
May 18, 2019·Investigative Ophthalmology & Visual Science·Theo G SeilerKarsten Koenig
Aug 22, 2020·Translational Vision Science & Technology·Arie L MarcovichAvigdor Scherz
Apr 2, 2021·Clinical Ophthalmology·Syed Bilal Hassan Zaidi, Wajid Ali Khan
Jul 31, 2021·Translational Vision Science & Technology·Brecken J BlackburnWilliam J Dupps

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