Evaluation of viability assays for anthocyanins in cultured cells

Phytochemical Analysis : PCA
Ingrid ElisiaDavid D Kitts

Abstract

The MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay is a widely accepted cytotoxicity assay which can produce inaccurate results due to possible interference with the antioxidant property of anthocyanins. Alternative methods to the MTT assay, such as BrdU (DNA-based) and CellTiter-Glo (ATP-based) assays were evaluated to assess anthocyanin cytotoxicity, derived from blackberry in LNCaP, MCF-7 and MDA-MB-453 cell lines. The standard cell counting method was the reference assay. Greater correlation of cell viability values following anthocyanin exposure was obtained from multiple cell lines with the alternative assays when compared with cell counting. MTT and cell counting results were not always correlated, albeit this was a function of cell type. In particular, poor correlations between cell counting and MTT procedures used to assess cytotoxicity of anthocyanins were observed in the MDA-MB-453 cell lines. Comparison of cytotoxicity derived from alternative assays and the MTT assays with the cell counting method was dependent on the assay procedure and the cell type. The LC(50) of blackberry crude extract ranged from 0.4 to 9.4 mg/mL between assays and across all cell lines, whereas a semi-purified antho...Continue Reading

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Citations

Aug 29, 2013·Drug Design, Development and Therapy·Qingli LiZongbing You
Feb 13, 2018·Journal of the Science of Food and Agriculture·Vitor C ChavesCláudia Mo Simões
Apr 12, 2019·International Journal of Molecular Sciences·Peter KubatkaDietrich Büsselberg
Oct 19, 2013·American Journal of Physiology. Gastrointestinal and Liver Physiology·Ingrid Elisia, David D Kitts
Jul 11, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Tong ZhangYing Chang
Feb 8, 2021·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Lorenzo Rivas-GarcíaCristina Sánchez-González
May 16, 2020·Materials Science & Engineering. C, Materials for Biological Applications·Roshanak KhandanlouHao Wang

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