Evaluation of virus reduction at a large-scale wastewater reclamation plant by detection of indigenous F-specific RNA bacteriophage genotypes

Environmental Technology
Suntae LeeHiroaki Tanaka

Abstract

Evaluating the reduction of virus load in water reclamation plants is important to ensuring the hygienic safety of the reclaimed water. A virus-spiking test is usually used to estimate virus reduction but is not practicable at large-scale plants. Thus, we evaluated virus reduction by ultrafiltration (UF) plus ultraviolet (UV) irradiation at a large-scale reclamation plant (1000 m3/d) by quantifying indigenous F-specific RNA bacteriophages (FRNAPHs). To detect the infectious FRNAPH, we used both plaque assay and integrated culture-reverse-transcription polymerase chain reaction combined with the most probable number assay, which can detect infectious FRNAPH genotypes. For comparison, we determined reductions of indigenous FRNAPHs and spiked MS2 at a small-scale pilot plant (10 m3/d) at the same time. Reductions by UF were not significantly different among the bacteriophages at pilot plants. This result suggests that indigenous bacteriophages could be used for evaluating virus reduction by UF at large-scale plants. Indigenous Genotype I (GI) FRNAPH showed the highest UV resistance, followed by GII, GIII, and GIV. The resistance of GI-FRNAPH was equivalent to that of spiked MS2. The reduction of the total infectious FRNAPHs determ...Continue Reading

References

Dec 1, 1991·Acta Crystallographica. Section B, Structural Science·K ValegårdT Unge
Sep 1, 1993·Applied and Environmental Microbiology·A H HavelaarY C Drost
May 15, 1996·Structure·R GolmohammadiL Liljas
Mar 2, 2002·Applied and Environmental Microbiology·Lester W SintonRobert J Davies-Colley
May 15, 2002·Journal of Virological Methods·Kirsten A MooijmanArie H Havelaar
Aug 30, 2003·Photochemistry and Photobiology·Ronald O RahnKeith R Lykke
Jan 1, 1908·The Journal of Hygiene·H Chick
Mar 17, 2012·Water Research·Nicolas BoudaudChristophe Gantzer
Jun 4, 2013·Water Research·Sihem JebriMoktar Hamdi
Mar 21, 2015·The Science of the Total Environment·Eiji HaramotoMikie Otagiri
Mar 18, 2016·Environment International·Daisuke SanoHiroyuki Katayama
May 22, 2016·Applied and Environmental Microbiology·Akihiko HataHiroaki Tanaka
Apr 30, 2017·Food and Environmental Virology·Suntae LeeHiroaki Tanaka

❮ Previous
Next ❯

Methods Mentioned

BETA
genotyping

Software Mentioned

GraphPad Prism
GraphPad

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.

Related Papers

Journal - Water Pollution Control Federation
R H YoungL S Lau
Zeitschrift Für Naturforschung. Teil B. Anorganische Chemie, Organische Chemie, Biochemie, Biophysik, Biologie
R KUHN, H WIEGANDT
© 2022 Meta ULC. All rights reserved