Evaluation of Xpert MTB/RIF assay for rapid molecular diagnosis of tuberculosis in a two-year period in Croatia

International Journal of Mycobacteriology
Ljiljana ZmakVera Katalinic Jankovic

Abstract

Mycobacterium tuberculosis remains a major global health problem and is currently killing 1.5 million people every year. One of the most important steps in tuberculosis control is the rapid and accurate laboratory diagnosis. The Xpert MTB/RIF assay is a novel molecular, easy-to-use assay, which can lead to tuberculosis identification in less than 2h. In this study, the Xpert MTB/RIF assay performance for rapid diagnosis of tuberculosis was evaluated in comparison with conventional culture methods; 361 pulmonary and extrapulmonary patient samples were collected between October 2010 and October 2012 and were analyzed at the National Reference laboratory for Mycobacteria, Zagreb, Croatia. For pulmonary samples the sensitivity and specificity were 86% and 100%, while for extrapulmonary samples the sensitivity and specificity were 75% and 99%, respectively. It was concluded that Xpert MTB/RIF assay has high sensitivity and specificity for both pulmonary and extrapulmonary specimens.

References

Sep 10, 2010·The New England Journal of Medicine·Catharina C BoehmeMark D Perkins
Jan 29, 2011·Journal of Clinical Microbiology·Doris HillemannElvira Richter
May 21, 2011·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Michael L Wilson
Oct 25, 2011·Lancet·Salmaan KeshavjeePaul E Farmer
May 25, 2012·Vaccine·UNKNOWN WHO Publication
Feb 27, 2013·The Cochrane Database of Systematic Reviews·Karen R SteingartNandini Dendukuri

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Citations

May 16, 2017·The Journal of Infection·Syed Beenish RufaiUNKNOWN TB Research Team
Aug 28, 2018·The Cochrane Database of Systematic Reviews·Mikashmi KohliKaren R Steingart
Jan 29, 2016·Journal of Clinical Microbiology·Inderpaul Singh SehgalRitesh Agarwal
Jun 8, 2019·The Cochrane Database of Systematic Reviews·David J HorneKaren R Steingart
Jan 31, 2019·Epidemiologic Reviews·Rodrigo Macedo CoutoEliseu Alves Waldman
Aug 28, 2020·Expert Review of Anti-infective Therapy·Vishal SharmaUsha Dutta
Nov 10, 2020·Tropical Medicine & International Health : TM & IH·Adrian V HernandezJose E Vidal
Jan 16, 2021·The Cochrane Database of Systematic Reviews·Mikashmi KohliKaren R Steingart
Nov 16, 2018·Indian Journal of Medical Microbiology·Sheetal BankarJayanthi Shastri

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