Event-specific qualitative polymerase chain reaction analysis for two T-DNA copies in genetically modified orange Petunia.

Plant Cell, Tissue and Organ Culture
Christian Haselmair-GoschHeidi Halbwirth

Abstract

In 2017, various orange coloured petunia on the market turned out to be genetically modified (GM) without an official authorization for commercialization. Sequence analysis suggested these undeclared plants most probably originated from a plant transformation experiment performed in the 1980s. For a deeper understanding how GM petunia entered classical breeding programmes worldwide, and whether they originated from a single source or not, we undertook a molecular genetic characterization of the T-DNA integration sites in different GM petunia cultivars and breeding lines. By means of genome walking, we isolated different T-DNA sequences, which are located at the junctions between the T-DNA(s) and the petunia DNA. Based on the results obtained we conclude that there are at least two T-DNA copies of different lengths. This is supported by Southern blot analysis. For T-DNA1, the 3'-junction sequence was isolated, whereas the 5'-junction remained unclear. In contrast, for T-DNA2, the 5'-junction sequence was isolated, whereas the sequence isolated from the 3'-region consists only of T-DNA, but did not include the junction from the T-DNA to the petunia DNA. We developed primers for event-specific PCRs and screened a set of three oran...Continue Reading

References

May 7, 2003·Analytical and Bioanalytical Chemistry·Arne Holst-JensenKnut G Berdal
Feb 16, 2012·Biotechnology Advances·Arne Holst-JensenDoerte Wulff
Nov 28, 2014·Nucleic Acids Research·Noe Fernandez-PozoLukas A Mueller
May 13, 2015·Genetics and Molecular Research : GMR·Y X ChuJ S Pan
Jun 4, 2016·Nature Plants·Aureliano BombarelyCris Kuhlemeier
Jun 26, 2017·Planta·Hany Bashandy, Teemu H Teeri
Feb 7, 2020·Horticulture Research·Anne-Laure BoutignyMathieu Rolland
Feb 1, 2021·Horticulture Research·Anne-Laure BoutignyMathieu Rolland

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Datasets Mentioned

BETA
MF521566
MN911270
MN911271

Methods Mentioned

BETA
transgenic
PCR
PCRs
genetic modifications
MinION
electrophoresis
phosphotransferase

Software Mentioned

Selecta One
BLAST
GenomeWalker

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