Everolimus quantification in peripheral blood mononuclear cells using ultra high performance liquid chromatography tandem mass spectrometry

Journal of Pharmaceutical and Biomedical Analysis
Francois Roullet-RenoleauChristine Fernandez

Abstract

A reliable ultra high performance liquid chromatography tandem mass spectrometry method has been developed for the determination of everolimus in human peripheral blood mononuclear cells (PBMCs). Protein precipitation was used for sample preparation. Analysis was performed on an UPLC Waters Acquity system. Chromatography was carried out using a cartridge column MassTrak TDM C18 (2.1×10 mm) with 0.1% formic acid and 2 mM of ammonium acetate in water and 0.1% formic acid and 2mM of ammonium acetate in methanol mixture as a mobile phase delivered at a flow rate of 0.4 mL/min in gradient mode. The assay was linear over a range of 0-12.5 ng/mL. The analysis of quality control samples at 2.5, 5.0 and 10.0 ng/mL demonstrated good precision with relative standard deviation of less than 15%. Recoveries at concentrations of 2.5, 5.0 and 10.0 ng/mL were all greater than 83%. The method was successfully applied to the analysis of everolimus in PBMCs from blood samples of transplant recipients.

References

Sep 1, 1995·The Journal of Clinical Investigation·T D BatiukP F Halloran
Jan 30, 2004·Clinical Pharmacokinetics·Gabriele I KirchnerMichael P Manns
Jul 2, 2004·Therapeutic Drug Monitoring·Michel EichelbaumMatthias Schwab
Jul 28, 2007·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Nicolas AnsermotSerge Rudaz

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Citations

Jun 25, 2013·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Florian LemaitreChristine Fernandez
Feb 24, 2015·Journal of Pharmaceutical and Biomedical Analysis·Debora PensiAntonio D'Avolio
Jul 30, 2016·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·A CapronP Wallemacq
Mar 25, 2019·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Sonali JainPinaki Sengupta

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