Abstract
3q21q26 syndrome, an acute myeloid leukemia (AML) or myelodysplastic syndrome (MDS) with chromosomal translocations or inversions between the bands 3q21 and 3q26, is frequently associated with dysmegakaryocytopoiesis and increased platelet counts at the initial diagnosis. Since the EVI1 gene at 3q26 is transcriptionally activated in 3q21q26 syndrome, we assessed the role of EVI1 gene expression in the abnormal megakaryocytic differentiation in 3q21q26 syndrome. RT-PCR analysis of various types of hematopoietic cells revealed that the EVI1 gene is expressed specifically in CD34(+) cells, megakaryocytes, and platelets. UT-7 is a human immature megakaryoblastic leukemia cell line with dependence for the growth on granulocyte-macrophage colony-stimulating factor (GM-CSF) (designated at UT-7/GM) and with a differentiation capacity to erythroid (UT-7/EPO) and megakaryocytic lineages (UT-7/TPO) by erythropoietin (EPO) and thrombopoietin (TPO), respectively. Among three UT-7 sublines, UT-7/GM, UT-7/EPO, and UT-7/TPO, expression of the EVI1 gene was detected at low levels in UT-7/GM and UT-7/EPO cells, but was detected at a higher level in UT-7/TPO cells. When UT-7/GM cells were cultured with TPO, the level of EVI1 expression was increa...Continue Reading
References
Jan 1, 1992·Molecular and Cellular Biology·K MorishitaJ N Ihle
May 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·K MorishitaJ N Ihle
Mar 1, 1990·Molecular and Cellular Biology·T MatsugiJ N Ihle
Jan 1, 1988·Molecular and Cellular Biology·M L MucenskiN G Copeland
Sep 9, 1988·Cell·K MorishitaJ N Ihle
May 1, 1995·Molecular and Cellular Biology·T TanakaH Hirai
Mar 1, 1995·Stem Cells·K L Block, M Poncz
Apr 26, 1994·Proceedings of the National Academy of Sciences of the United States of America·G NuciforaJ D Rowley
Jul 1, 1993·Molecular and Cellular Biology·R DelwelJ N Ihle
Jul 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·B L KreiderJ N Ihle
Jan 1, 1993·Molecular and Cellular Biology·V LemarchandelP H Roméo
Jul 4, 1998·Oncogene·Y HamazakiT Nagasawa
Nov 11, 1999·Leukemia·S SitailoG Nucifora
Citations
Apr 2, 2010·Leukemia·B V BalgobindM M van den Heuvel-Eibrink
Jan 19, 2012·Molecular Therapy : the Journal of the American Society of Gene Therapy·Luca BiascoAlessandro Aiuti
Jul 17, 2009·Journal of Leukocyte Biology·Torsten A KonradRotraud Wieser
Mar 5, 2013·PloS One·Anna RommerRotraud Wieser
Apr 25, 2015·Leukemia & Lymphoma·Maria E CardonaH Jose Arteaga
Oct 27, 2015·International Journal of Oncology·Xiaofen YuanGuosheng Jiang
Sep 15, 2005·British Journal of Haematology·Anna KilbeyChristopher Bartholomew
Dec 9, 2014·Cell Cycle·Birgit SteinmetzRotraud Wieser
Jun 15, 2011·Blood·Yi ZhangArchibald S Perkins
Nov 19, 2014·PloS One·Nathalie Delesque-TouchardFrançoise Bono
Apr 24, 2009·Cancer Science·Susumu Goyama, Mineo Kurokawa
May 13, 2005·The EMBO Journal·Hiromi YuasaKazuhiro Morishita
Apr 18, 2015·Journal of Hematology & Oncology·Gerwin HellerRotraud Wieser
Jun 13, 2012·Oncogene·M MaicasM D Odero
Apr 8, 2015·Nature Communications·William TapperNicholas C P Cross
Jan 15, 2013·Journal of Thrombosis and Haemostasis : JTH·M R Tijssen, C Ghevaert
Mar 24, 2021·Blood Cancer Journal·Christine BirdwellKapil N Bhalla
May 27, 2008·Biochemistry·Dominik SpensbergerRuud Delwel
May 18, 2007·Gene·Rotraud Wieser