Eviction of linker histone H1 by NAP-family histone chaperones enhances activated transcription

Epigenetics & Chromatin
Qian ZhangJennifer K Nyborg

Abstract

In the Metazoan nucleus, core histones assemble the genomic DNA to form nucleosome arrays, which are further compacted into dense chromatin structures by the linker histone H1. The extraordinary density of chromatin creates an obstacle for accessing the genetic information. Regulation of chromatin dynamics is therefore critical to cellular homeostasis, and histone chaperones serve as prominent players in these processes. In the current study, we examined the role of specific histone chaperones in negotiating the inherently repressive chromatin structure during transcriptional activation. Using a model promoter, we demonstrate that the human nucleosome assembly protein family members hNap1 and SET/Taf1β stimulate transcription in vitro during pre-initiation complex formation, prior to elongation. This stimulatory effect is dependent upon the presence of activators, p300, and Acetyl-CoA. We show that transcription from our chromatin template is strongly repressed by H1, and that both histone chaperones enhance RNA synthesis by overcoming H1-induced repression. Importantly, both hNap1 and SET/Taf1β directly bind H1, and function to enhance transcription by evicting the linker histone from chromatin reconstituted with H1. In vivo s...Continue Reading

References

Dec 1, 1992·Genes & Development·G E CrostonJ T Kadonaga
May 10, 1996·The Journal of Biological Chemistry·M LiZ Damuni
Nov 5, 1997·Journal of Molecular Biology·K LugerT J Richmond
Jun 18, 1999·Methods in Enzymology·K LugerT J Richmond
Dec 12, 2001·Molecular and Cellular Biology·Sara A GeorgesPaul J Laybourn
Dec 12, 2003·The Journal of Biological Chemistry·Xu Lu, Jeffrey C Hansen
Jan 10, 2004·Methods in Enzymology·Dmitry V Fyodorov, James T Kadonaga
Apr 22, 2004·The Journal of Biological Chemistry·Robert SchneiderTony Kouzarides
Jul 9, 2004·International Journal of Cancer. Journal International Du Cancer·Shoko SaitoKyosuke Nagata
Nov 2, 2004·The Journal of Biological Chemistry·Young-Jun ParkKarolin Luger
Jan 6, 2005·Molecular and Cellular Biology·Matthew J GambleRobert P Fisher
Mar 8, 2005·Molecular Cell·Michael BustinJae-Hwan Lim
Jun 2, 2005·Proceedings of the National Academy of Sciences of the United States of America·Keishi ShintomiKeita Ohsumi
Aug 3, 2005·Biochemical and Biophysical Research Communications·Zoe KaretsouThomais Papamarcaki
Aug 18, 2005·The Journal of Biological Chemistry·J Felix KepertKarsten Rippe
Feb 24, 2006·Proceedings of the National Academy of Sciences of the United States of America·Yahli LorchRoger D Kornberg
Mar 21, 2006·The Journal of Biological Chemistry·Isabelle LemassonJennifer K Nyborg
Aug 29, 2006·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Young-Jun Park, Karolin Luger
Dec 21, 2006·Nucleic Acids Research·Kohsuke KatoKyosuke Nagata
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Shinsuke MutoMasami Horikoshi
May 29, 2007·Nature Structural & Molecular Biology·Matthew J Gamble, Robert P Fisher

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Citations

Mar 3, 2017·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Kaori KajitaniKyosuke Nagata
Mar 19, 2020·Journal of Cell Science·Imke K MandemakerJurgen A Marteijn
Apr 1, 2016·Epigenetics & Chromatin·Arvind Panday, Anne Grove
Jul 21, 2020·Epigenetics : Official Journal of the DNA Methylation Society·Nandini Karthik, Reshma Taneja
Jan 6, 2021·Genes & Development·Laura Prendergast, Danny Reinberg
Dec 29, 2020·The Plant Journal : for Cell and Molecular Biology·Karolína KolářováMartina Dvořáčková
May 7, 2021·Annual Review of Biophysics·Ping ChenGuohong Li
Jun 12, 2021·Biochimica Et Biophysica Acta. Reviews on Cancer·E C DacolA P Lepique
Sep 23, 2021·Journal of Biochemistry·Mitsuhiro YonedaTakashi Ito

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Methods Mentioned

BETA
acetylation
in vitro transcription
histone acetylation
pull-down
transfection
PCR
electrophoresis

Software Mentioned

ImageQuant
SlideBook ( Intelligent Imaging Innovations )

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