Evidence against a direct role for the Upf proteins in frameshifting or nonsense codon readthrough

RNA
Jason W Harger, Jonathan D Dinman

Abstract

The Upf proteins are essential for nonsense-mediated mRNA decay (NMD). They have also been implicated in the modulation of translational fidelity at viral frameshift signals and premature termination codons. How these factors function in both mRNA turnover and translational control remains unclear. In this study, mono- and bicistronic reporter systems were used in the yeast Saccharomyces cerevisae to differentiate between effects at the levels of mRNA turnover and those at the level of translation. We confirm that upfDelta mutants do not affect programmed frameshifting, and show that this is also true for mutant forms of eIF1/Sui1p. Further, bicistronic reporters did not detect defects in translational readthrough due to deletion of the UPF genes, suggesting that their function in termination is not as general a phenomenon as was previously believed. The demonstration that upf sui1 double mutants are synthetically lethal demonstrates an important functional interaction between the NMD and translation initiation pathway.

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Citations

Jun 2, 2005·Reviews of Physiology, Biochemistry and Pharmacology·S RospertY Dubaquié
Jul 28, 2005·Molecular Genetics and Genomics : MGG·Sergey KiparisovJonathan D Dinman
Sep 27, 2006·Molecular and Cellular Biology·Rasa Rakauskaite, Jonathan D Dinman
Feb 12, 2008·Nucleic Acids Research·Arturas MeskauskasJonathan D Dinman
Mar 29, 2008·RNA·Lara AjamianAndrew J Mouland
Aug 19, 2008·Journal of Bacteriology·Tit-Yee WongJong-Kang Liu
Aug 14, 2010·Nucleic Acids Research·Michael H J Rhodin, Jonathan D Dinman
Jun 19, 2015·Journal of Environmental Science and Health. Part C, Environmental Carcinogenesis & Ecotoxicology Reviews·Tit-Yee Wong, Steve D Schwartzbach
Oct 20, 2010·FEMS Yeast Research·Laure BidouOlivier Namy
Jul 9, 2020·Biomolecules·Daria Lavysh, Gabriele Neu-Yilik
Oct 23, 2020·Biological Reviews of the Cambridge Philosophical Society·Martine Palma, Fabrice Lejeune

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