Evidence against a role of mouse, rat, and two cloned human t1alpha isoforms as a water channel or a regulator of aquaporin-type water channels.

American Journal of Respiratory Cell and Molecular Biology
T MaA S Verkman

Abstract

T1alpha is a protein of unknown function that is expressed at the plasma membrane in epithelia involved in fluid transport, including type I alveolar epithelial cells, choroid plexus, and ciliary epithelium. The purpose of this study was to test the hypothesis that T1alpha functions as a water channel or a regulator of aquaporin-type water channels that are coexpressed with T1alpha. Two complementary DNAs (cDNAs) (hT1alpha-1 and hT1alpha-2) encoding human isoforms of T1alpha were cloned by homology to the rat T1alpha coding sequence. The cDNAs encoded 164 (hT1alpha-1) and 162 (hT1alpha-2) amino acid proteins with high homology to rat T1alpha in a putative membrane-spanning domain. hT1alpha-1 transcripts of 2. 6 and 1.4 kb were detected in human lung, heart, and skeletal muscle, and a single hT1alpha-2 transcript of 1.2 kb was detected in human lung. Rat and mouse T1alpha were isolated by reverse transcription-polymerase chain reaction and confirmed by DNA sequence analysis. Expression of mouse, rat, and human T1alpha isoforms in Xenopus oocytes did not increase osmotic water permeability (Pf) above that in water-injected oocytes, nor was there an effect of protein kinase A or C activation; Pf was increased > 10-fold in positive...Continue Reading

References

Nov 27, 1992·Science·H HasegawaA S Verkman
Mar 19, 1992·Nature·M PaulmichlD Clapham
Apr 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J FischbargO M Rosen
Apr 1, 1994·The American Journal of Physiology·H HasegawaA S Verkman
May 24, 1994·Proceedings of the National Academy of Sciences of the United States of America·H G FolkessonA S Verkman
May 9, 1995·Proceedings of the National Academy of Sciences of the United States of America·A FrigeriA S Verkman
Aug 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·S NielsenP Agre
May 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·C BondyP Agre
Feb 1, 1996·The Journal of Clinical Investigation·H G FolkessonA S Verkman
Jun 1, 1996·American Journal of Respiratory Cell and Molecular Biology·M C WilliamsA Wetterwald
Jan 1, 1996·The American Journal of Physiology·A S VerkmanJ Farinas
Nov 12, 1996·Proceedings of the National Academy of Sciences of the United States of America·D D LooE M Wright
Nov 1, 1996·American Journal of Respiratory Cell and Molecular Biology·F UmenishiA S Verkman
Sep 1, 1997·The Journal of Clinical Investigation·E P CarterA S Verkman

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Citations

Feb 18, 2011·Pflügers Archiv : European journal of physiology·Bayram EdemirThomas Weide
Jan 18, 2006·Virchows Archiv : an International Journal of Pathology·Junji ShibaharaMasashi Fukayama
Mar 16, 2007·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Marc HinterbergerHolger Moch
Jun 2, 2006·Molecular and Cellular Biology·Keqin ZhangLynda F Bonewald
Feb 15, 2014·BioMed Research International·Emily M McNellisIkechukwu I Ekekezie
Apr 3, 2001·American Journal of Respiratory and Critical Care Medicine·E D Crandall, M A Matthay
Mar 28, 2008·American Journal of Respiratory Cell and Molecular Biology·Jeff N VanderbiltLeland G Dobbs
Nov 13, 2002·Annual Review of Physiology·Mary C Williams
Jul 12, 2005·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Yukinari KatoMotoki Osawa
Jan 17, 2009·The International Journal of Biochemistry & Cell Biology·Ester Martín-VillarJaime Renart
Oct 30, 2004·International Journal of Cancer. Journal International Du Cancer·Ester Martín-VillarMiguel Quintanilla
May 4, 2006·Advances in Anatomic Pathology·Nelson G Ordóñez
Aug 19, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Telugu A NarasarajuLin Liu
Jul 5, 2017·Cancer Metastasis Reviews·Ai TakemotoNaoya Fujita
Apr 27, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·A S VerkmanY Song
Dec 9, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Marion GrögerPeter Petzelbauer
Nov 20, 1998·The American Journal of the Medical Sciences·A S Verkman

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