Evidence against necessary phosphorylation during hexose transport in Aspergillus nidulans.

Journal of Bacteriology
C E Brown, A H Romano


The transport of 2-deoxy-d-glucose, a nonmetabolizable glucose analogue, into Aspergillus nidulans against a concentration gradient does not appear to require phosphorylation, despite the high levels of sugar phosphates accumulated rapidly within the cell. Two other deoxy analogues of d-glucose, 6-deoxy-d-glucose and 1,5-anhydro-d-glucitol (1-deoxy-d-glucose), although they lack the C-6 and the C-1 hydroxyl groups, respectively, and thus cannot be phosphorylated in those positions, still competitively inhibit the entry of 2-deoxy-d-glucose. Moreover, 6-deoxy-d-glucose can be concentrated against a gradient within the cell without the accumulation of 6-deoxy-d-glucose-phosphate. d-Galactose shows an intracellular ratio of free to phosphorylated sugar similar to that found for 2-deoxy-d-glucose in cells that have galactokinase, but no sugar phosphates are found in a galactokinaseless mutant strain. These data suggest that intracellular kinases are responsible for the sugar phosphate pool; and indeed, a kinase capable of phosphorylating 2-deoxy-d-glucose has been demonstrated. Finally, experiments on the kinetics of labeling of intracellular free sugar and sugar phosphate pools with (14)C-2-deoxy-d-glucose show that radioactivity ...Continue Reading


Apr 7, 1967·Biochemical and Biophysical Research Communications·S TanakaE C Lin
Nov 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·R D SimoniS Roseman
Apr 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·S Tanaka, E C Lin
Mar 28, 1966·Biochimica Et Biophysica Acta·H H Winkler
Jul 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·E P Kennedy, G A Scarborough
Jun 24, 1968·Biochimica Et Biophysica Acta·A H Romano, H L Kornberg
Nov 5, 1968·Biochimica Et Biophysica Acta·J van Steveninck
Jan 1, 1953·Advances in Genetics·G PONTECORVOA W J BUFTON
Jun 1, 1956·Biochimica Et Biophysica Acta·R K CRANE, S M KRANE
Oct 1, 1964·Proceedings of the National Academy of Sciences of the United States of America·W KUNDIGS ROSEMAN


Dec 15, 1977·Experientia·J D Desai, V V Modi
Aug 1, 1976·The Journal of protozoology·F W Schaefer, A J Mukkada
May 1, 1974·The Journal of protozoology·A J MukkadaC Neu
Sep 3, 2013·Applied and Environmental Microbiology·Kimran HayerDavid B Archer
Dec 3, 1973·European Journal of Biochemistry·M A TarshisH I Bourd
Jan 1, 1971·Molecular & General Genetics : MGG·M V Elorza, H N Arst
Nov 1, 1973·European Journal of Biochemistry·B Sabater, C Asensio
Nov 1, 1970·Journal of Bacteriology·A H RomanoT D McDowell
May 1, 1971·Journal of Bacteriology·R P Schneider, W R Wiley
Jun 1, 1984·Applied and Environmental Microbiology·R L Buchanan, D F Lewis
May 26, 1977·Biochimica Et Biophysica Acta·S A Meredith, A H Romano
Jul 6, 1971·Biochimica Et Biophysica Acta·E Komor, W Tanner
Aug 1, 1990·Applied and Environmental Microbiology·Rogélio L Brandão, Maria C Loureiro-Dias
Dec 14, 1976·Biochimica Et Biophysica Acta·P W Postma, S Roseman
Mar 5, 1976·Biochimica Et Biophysica Acta·P W Royt, A M MacQuillan

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Spatio-Temporal Regulation of DNA Repair

DNA repair is a complex process regulated by several different classes of enzymes, including ligases, endonucleases, and polymerases. This feed focuses on the spatial and temporal regulation that accompanies DNA damage signaling and repair enzymes and processes.

Glut1 Deficiency

Glut1 deficiency, an autosomal dominant, genetic metabolic disorder associated with a deficiency of GLUT1, the protein that transports glucose across the blood brain barrier, is characterized by mental and motor developmental delays and infantile seizures. Follow the latest research on Glut1 deficiency with this feed.

Hereditary Sensory Autonomic Neuropathy

Hereditary Sensory Autonomic Neuropathies are a group of inherited neurodegenerative disorders characterized clinically by loss of sensation and autonomic dysfunction. Here is the latest research on these neuropathies.

Separation Anxiety

Separation anxiety is a type of anxiety disorder that involves excessive distress and anxiety with separation. This may include separation from places or people to which they have a strong emotional connection with. It often affects children more than adults. Here is the latest research on separation anxiety.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.