Evidence for a 1 alpha,25-dihydroxyvitamin D3 receptor/binding protein in a membrane fraction isolated from a chick tibial fracture-healing callus

Biochemical and Biophysical Research Communications
A KatoAnthony W Norman

Abstract

Previous biological studies have implicated two vitamin D metabolites, 1 alpha,25(OH)2-vitamin D3[1 alpha,25(OH)2-D3] and 24R,25(OH)2-vitamin D3 [24R,25(OH)2D3] in the process of skeletal fracture-healing. While a nuclear receptor for 1 alpha,25(OH)2D3 is known to be present in osteoblast and absent in osteoclast cell lines, no systematic study has been carried out on the callus tissue which is formed during fracture-healing. The present report shows that a binding protein/receptor for 1 alpha,25(OH)2D3 resides both in a postnuclear membrane fraction and in a high speed cytosol fraction of the callus tissue obtained 10 days after imposition of a tibial fracture. The dissociation constant, KD, for 1 alpha,25(OH)2D3 was 0.83 +/- 0.34 M and 0.66 +/- 0.38 nM respectively, for the membrane and cytosol fractions. Results from a panel of steroid competition assays indicate that both receptor/binding proteins greatly prefer 1 alpha-hydroxylated ligands as compared to 1 alpha-deoxy or 24-hydroxylated ligands. The presence of 1 alpha,25(OH)2D3 receptors in the fracture-healing callus is consistent with the known biological effects of the metabolite on the fracture-healing process.

References

Jan 1, 1990·The Journal of Bone and Joint Surgery. British Volume·C LidorS Edelstein
Dec 1, 1988·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·P P Minghetti, A W Norman
Jun 1, 1987·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·I Nemere, A W Norman
Apr 1, 1987·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·C LidorS Edelstein
Jun 1, 1995·The Journal of Steroid Biochemistry and Molecular Biology·W H OkamuraA W Norman
Nov 1, 1994·Nutrition Reviews·S S Hannah, A W Norman
Apr 1, 1997·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·E G Seo, A W Norman

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Citations

Jan 3, 2013·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Adam D M BriggsAdrian R Martineau
Sep 3, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Joost G J HoenderopRené J M Bindels
Jan 26, 2021·Journal of Endocrinological Investigation·J VaculikJ J Stepan

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