PMID: 2493809Mar 28, 1989Paper

Evidence for a bidirectional prostaglandin endoperoxide shunt between platelets and the bovine coronary artery

Biochimica Et Biophysica Acta
P R MayeuxD B McNamara

Abstract

While platelets have been shown to be capable of supplying prostaglandin (PG) H2 to endothelial cells in culture for PGI2 synthesis, endothelial cells have been shown unable to supply PGH2 to platelets for thromboxane (TX) A2 synthesis. We incubated rings of the bovine coronary artery (BCAR) with human platelets treated with aspirin (to inhibit cyclooxygenase) or CGS 13080 (to inhibit TXA2 synthase) in the presence of 20 microM arachidonic acid. BCAR, with damaged endothelium, produced significantly less PGI2 than that with intact endothelium. However, co-incubation with CGS 13080-treated platelets resulted in an increase in PGI2 independent of endothelium, demonstrating a shunt of PGH2 from platelets to BCAR. Co-incubation of BCAR with aspirin-treated platelets resulted in a net increase in TXA2 demonstrating a shunt of PGH2 from BCAR to platelets. Employing [14C]PGH2 as substrate, BCAR with and without intact endothelium produced similar amounts of 6-keto-[14C]PGF1 alpha. Likewise, homogenates (50 micrograms protein) of intimal and subintimal regions of BCAR and BCAR converted similar amounts of PGH2 to 6-keto-PGF1 alpha. These data suggest that vascular production of PGH2 is more dependent on an intact endothelium than is th...Continue Reading

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Citations

Sep 2, 1999·European Journal of Obstetrics, Gynecology, and Reproductive Biology·T J SchneiderH C Wallenburg
Nov 14, 2002·British Journal of Pharmacology·J P De La CruzF Sánchez de La Cuesta
Nov 9, 2000·The Journal of Experimental Medicine·B K KubataO Hayaishi
Feb 1, 1997·Prostaglandins, Leukotrienes, and Essential Fatty Acids·M Zou, C Anges
May 9, 2009·Arteriosclerosis, Thrombosis, and Vascular Biology·M Dolores SalvadoJuan Miguel Redondo
Aug 8, 2012·Nature Reviews. Rheumatology·Eric BoilardPeter A Nigrovic
Mar 1, 1996·Journal of Cardiac Surgery·C D IanuzzoR E Klabunde
Mar 2, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Eric BoilardDavid M Lee
Jan 1, 1991·Prostaglandins, Leukotrienes, and Essential Fatty Acids·E A BurchD B McNamara

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