PMID: 6852354Jan 1, 1983

Evidence for a biologically active, carrier-bound form of non-suppressible insulin-like activity (NSILA) in human serum

The International Journal of Biochemistry
H J Cornell, A C Herington

Abstract

1. Quantitative gel filtration of Cohn fraction IV-1 has shown that high MW NSILA recovered under "neutral" conditions (pH 5.5) was greater in magnitude (approximately X 2) than acid-stable high MW NSILA obtained under acid conditions. 2. Recombination, at neutral poH, of high and low MW NSILA, obtained by previous acid gel filtration, gave quantitative recovery of activity which was solely of high MW. 3. These data indicate that a biologically active, and dissociable high MW form of NSILA exists in serum. 4. Con A-Sepharose affinity chromatography gave one unbound NSILA fraction and two bound fractions, which both gave high and low MW activity after acid chromatography. 5. This study provides the first evidence for a carrier-bound form of low MW NSILA which retains biological activity.

References

Nov 1, 1979·Molecular and Cellular Endocrinology·R C FranklinA C Herington
Dec 1, 1978·The Journal of Clinical Endocrinology and Metabolism·B I PosnerS Goldstein
Feb 14, 1979·Biochemical and Biophysical Research Communications·W Y FujimotoP L Poffenbarger
May 15, 1978·FEBS Letters·E Rinderknecht, R E Humbel
Jan 1, 1977·The Journal of Clinical Endocrinology and Metabolism·U KaufmannE R Froesch
Nov 1, 1976·The Journal of Clinical Endocrinology and Metabolism·R C FranklinD P Cameron
Dec 1, 1975·The Journal of Clinical Investigation·P L Poffenbarger
Jul 1, 1980·The Journal of Clinical Endocrinology and Metabolism·R W Furlanetto

Citations

Jan 1, 1983·The International Journal of Biochemistry·A C HeringtonA D Kuffer
Jan 1, 1985·The International Journal of Biochemistry·H J CornellA C Herington
Jan 1, 1989·Acta Paediatrica Scandinavica. Supplement·J Gustafsson
Jun 1, 1988·Diabetes/metabolism Reviews·M Press

Related Concepts

concanavalin A-sepharose
Chromatography, Affinity
Molecular Sieve Chromatography
Hydrogen-Ion Concentration
NSILA-S
Sepharose

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