Evidence for a biphasic mode of respiratory syncytial virus transmission in permissive HEp2 cell monolayers

Virology Journal
Tra Nguyen HuongRichard J Sugrue

Abstract

During respiratory syncytial virus (RSV) infection filamentous virus particles are formed on the cell surface. Although the virus infectivity remains cell-associated, low levels of cell-free virus is detected during advanced infection. It is currently unclear if this cell-free virus infectivity is due to a low-efficiency specific cell-release mechanism, or if it arises due to mechanical breakage following virus-induced cell damage at the advanced stage of infection. Understanding the origin of this cell-free virus is a prerequisite for understanding the mechanism of RSV transmission in permissive cells. In this study we describe a detailed examination of RSV transmission in permissive HEp2 cell monolayers. HEp2 cell monolayers were infected with RSV using a multiplicity of infection of 0.0002, and the course of infection monitored over 5 days. The progression of the virus infection within the cell monolayers was performed using bright-field microscopy to visualise the cell monolayer and immunofluorescence microscopy to detect virus-infected cells. The cell-associated and cell-free virus infectivity were determined by virus plaque assay, and the virus-induced cell cytotoxicity determined by measuring cell membrane permeability a...Continue Reading

References

Jan 1, 1969·Archiv für die gesamte Virusforschung·S Levine, R Hamilton
May 5, 2001·Virology·T L GowerB S Graham
May 31, 2002·Nature·Pradeep K SinghMichael J Welsh
Jan 15, 2003·Journal of Virology·Lewis H McCurdy, Barney S Graham
Jan 22, 2004·The Journal of Experimental Medicine·Clare JollyQuentin J Sattentau
May 7, 2004·Proceedings of the National Academy of Sciences of the United States of America·Yuan GaoYi Zheng
Dec 5, 2006·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Joyce E JohnsonBarney S Graham
Jan 16, 2007·Applied and Environmental Microbiology·Stephanie A Boone, Charles P Gerba
Jun 15, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·Divyendu SinghFarhad Imani
Jun 15, 2007·Toxicologic Pathology·Susan Elmore
Aug 19, 2007·Archives of Virology·C CarromeuA M Ventura
Sep 9, 2010·Cell Communication and Signaling : CCS·Matteo Parri, Paola Chiarugi
Mar 5, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Sandra Hervas-StubbsIgnacio Melero
Dec 21, 2012·Future Microbiology·Fyza Y Shaikh, James E Crowe
Jun 19, 2013·Proceedings of the National Academy of Sciences of the United States of America·Lassi LiljeroosSarah Jane Butcher
Nov 30, 2014·Antiviral Research·Muhammad Raihan JumatRichard J Sugrue

❮ Previous
Next ❯

Methods Mentioned

BETA
fluorescence microscopy
confocal microscopy
GTPase
biopsy
electron microscopy
FCS

Software Mentioned

Control

Related Concepts

Related Feeds

Advanced Imaging of Cellular Signaling

Cell signaling is a vital mechanism for communication within cells and outside with the environment. Several different signaling pathways have been found and advanced imaging techniques are being developed to visualize the molecules involved in these signaling pathways. Find the latest research in advanced imaging of cellular signaling here.

© 2022 Meta ULC. All rights reserved