Evidence for a carbohydrate-binding module (CBM) of Tannerella forsythia NanH sialidase, key to interactions at the host-pathogen interface

The Biochemical Journal
A M FreyGraham P Stafford

Abstract

Bacterial sialidases cleave terminal sialic acid from a variety of host glycoproteins, and contribute to survival and growth of many human-dwelling bacterial species, including various pathogens. Tannerella forsythia, an oral, Gram-negative, fastidious anaerobe, is a key organism in periodontal disease and possesses a dedicated sialic acid utilisation and scavenging (nan) operon, including NanH sialidase. Here, we describe biochemical characterisation of recombinant NanH, including its action on host-relevant sialoglycans such as sialyl Lewis A and sialyl Lewis X (SLeA/X), and on human cell-attached sialic acids directly, uncovering that it is a highly active broad specificity sialidase. Furthermore, the N-terminal domain of NanH was hypothesised and proved to be capable of binding to a range of sialoglycans and non-sialylated derivatives with Kd in the micromolar range, as determined by steady-state tryptophan fluorescence spectroscopy, but it has no catalytic activity in isolation from the active site. We consider this domain to represent the founding member of a novel subfamily of carbohydrate-binding module (CBM), involved in glycosidase-ligand binding. In addition, we created a catalytically inactive version of the NanH en...Continue Reading

References

Feb 1, 1992·Infection and Immunity·J E GalenJ B Kaper
Jan 1, 1991·Archives of Oral Biology·F M EggertM Goldner
Jan 1, 1985·Journal of Periodontal Research·M Bickel, G Cimasoni
Sep 1, 1993·Molecular Microbiology·P RoggentinE R Vimr
Jul 1, 1996·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·E R PietrzakL J Walsh
Nov 14, 1997·Journal of Biochemical and Biophysical Methods·E L RomeroS Alonso
Mar 12, 1998·Journal of Clinical Periodontology·S S SocranskyR L Kent
Jul 31, 1999·Applied and Environmental Microbiology·J M VroomC Allison
Sep 4, 1999·Journal of Cancer Research and Clinical Oncology·J RenkonenR Renkonen
Mar 9, 2000·Journal of Medical Microbiology·H L ByersD Beighton
Oct 6, 2000·Journal of Biomolecular Structure & Dynamics·S NarasimhamurthyM J Levine
Jun 1, 2001·The Journal of Steroid Biochemistry and Molecular Biology·A S BhatnagarW R Miller
Oct 2, 2003·Current Opinion in Cell Biology·John B Lowe
Jul 1, 2004·The Journal of Biological Chemistry·Ibrahim MoustafaGarry Taylor
Jul 4, 2007·Pathology Oncology Research : POR·María V CroceAmada Segal-Eiras
Jan 11, 2008·Advances in Experimental Medicine and Biology·C Kunz, S Rudloff
Mar 4, 2008·Journal of Proteome Research·Alessio CeroniStuart M Haslam
Jul 16, 2008·Current Opinion in Chemical Biology·Alejandro Buschiazzo, Pedro M Alzari
Oct 7, 2008·Journal of Molecular Biology·Guogang XuGarry L Taylor
Mar 24, 2009·Journal of Bacteriology·Hayley ThompsonArthur H F Hosie
Jul 1, 2009·Infection and Immunity·Salvador Almagro-Moreno, E Fidelma Boyd
Jun 4, 2010·Journal of Clinical Microbiology·Calum JohnstonTim J Mitchell
Aug 18, 2010·Periodontology 2000·Ashu Sharma
Nov 17, 2010·Infection and Immunity·Kiyonobu HonmaAshu Sharma
Jan 20, 2011·Journal of the American Chemical Society·Guogang XuGarry L Taylor
Sep 3, 2011·Microbiology·Sumita RoyGraham P Stafford

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Citations

May 12, 2020·Journal of Dental Research·S Ji, Y Choi
Jan 17, 2020·International Journal of Biological Macromolecules·Sabina LipničanováStanislav Miertuš
Mar 11, 2021·Periodontology 2000·Nicholas S JakubovicsFabian Cieplik

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