PMID: 8944648Nov 1, 1996Paper

Evidence for a central role of transcription in the timing mechanism of a circadian clock

The American Journal of Physiology
S B KhalsaG D Block

Abstract

The retinal circadian clock in the isolated in vitro eye of the marine mollusc Bulla gouldiana exhibits a phase-dependent requirement for transcription. The transcription-sensitive phase extends through most of the subjective day and therefore is substantially longer than the previously reported translation-sensitive phase. Lower concentrations of transcription inhibitors yield a significant dose-dependent lengthening of circadian period. Clock motion can be stopped by a high concentration of the transcription inhibitor 5,6-dichlorobenz-imidazole riboside (DRB) when applied during the sensitive phase; after withdrawal of the inhibitor, motion resumes from the phase at which it was stopped. In a double-pulse experiment, phase shifts to light pulses applied after DRB pulses, and not during the translation-sensitive phase, indicate that the inhibition of transcription has immediate effects on the phase of the clock. These data suggest that DRB-induced phase shifts are independent of translation, which implies that the rate of transcription itself plays a significant role in the mechanism underlying the generation of the circadian cycle.

References

Nov 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·S B KhalsaG D Block
Feb 1, 1992·Journal of Comparative Physiology. A, Sensory, Neural, and Behavioral Physiology·C S ColwellG D Block
Dec 1, 1988·Journal of Comparative Physiology. A, Sensory, Neural, and Behavioral Physiology·S B Khalsa, G D Block
Dec 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·S PenmanM Penman
Nov 1, 1995·Neuron·S M Reppert, I Sauman
Jan 1, 1993·International Review of Cytology·G D BlockM Guesz
Nov 3, 1995·Cell·S A Kay, A J Millar
Oct 1, 1995·Current Opinion in Genetics & Development·M Rosbash

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Citations

Jun 18, 1999·Chronobiology International·E D Herzog, G D Block
Apr 13, 2000·Journal of Biological Rhythms·T L Page
Apr 9, 1999·Journal of Biological Rhythms·M A SloanA Eskin
Dec 11, 2002·Journal of Neurochemistry·Samer HattarArnold Eskin
Dec 19, 2003·Chronobiology International·Carlos RossiniJ Woodland Hastings
Jan 29, 2011·Nature·John S O'NeillAndrew J Millar
Dec 15, 2018·The European Journal of Neuroscience·Helen C Causton
May 25, 1999·Progress in Neurobiology·J Falcón
May 3, 2011·Current Biology : CB·Gerben van OoijenAndrew J Millar

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