Evidence for a credit-card-swipe mechanism in the human PC floppase ABCB4.

Structure
Martin PrescherLutz Schmitt

Abstract

ABCB4 is described as an ATP-binding cassette (ABC) transporter that primarily transports lipids of the phosphatidylcholine (PC) family but is also capable of translocating a subset of typical multidrug-resistance-associated drugs. The high degree of amino acid identity of 76% for ABCB4 and ABCB1, which is a prototype multidrug-resistance-mediating protein, results in ABCB4's second subset of substrates, which overlap with ABCB1's substrates. This often leads to incomplete annotations of ABCB4, in which it was described as exclusively PC-lipid specific. When the hydrophilic amino acids from ABCB4 are changed to the analogous but hydrophobic ones from ABCB1, the stimulation of ATPase activity by 1,2-dioleoyl-sn-glycero-3-phosphocholine, as a prime example of PC lipids, is strongly diminished, whereas the modulation capability of ABCB1 substrates remains unchanged. This indicates two distinct and autonomous substrate binding sites in ABCB4.

References

Nov 11, 1976·Biochimica Et Biophysica Acta·R L Juliano, V Ling
Nov 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·S Henikoff, J G Henikoff
Nov 30, 1988·Gene·A M van der BliekP Borst
Mar 30, 1971·Biochemistry·R D Kornberg, H M McConnell
May 25, 1972·Biochimica Et Biophysica Acta·J Rosing, E C Slater
Apr 4, 2001·Naunyn-Schmiedeberg's Archives of Pharmacology·C Pauli-MagnusM F Fromm
Oct 11, 2003·The Journal of Biological Chemistry·Marwan K Al-ShawiRobert A Figler
May 12, 2005·Journal of the American Chemical Society·Alejandro CrespoAdrian E Roitberg
Sep 21, 2006·Journal of the American Chemical Society·D Peter Tieleman, Siewert-Jan Marrink
Nov 30, 2006·Nucleic Acids Research·Walter PirovanoJaap Heringa
Jun 23, 2007·Nucleic Acids Research·K Anton FeenstraJaap Heringa
Aug 29, 2007·Current Opinion in Structural Biology·Kaspar HollensteinKaspar P Locher
Mar 21, 2008·Journal of Computational Chemistry·Sunhwan JoWonpil Im
Jan 29, 2011·BMC Structural Biology·Michał RostkowskiJan H Jensen
Apr 19, 2011·Journal of Computational Chemistry·Naveen Michaud-AgrawalOliver Beckstein
Sep 6, 2011·Nucleic Acids Research·Mikhail A LomizeAndrei L Lomize
Oct 25, 2012·PLoS Computational Biology·Eva ChovancovaJiri Damborsky
Jan 12, 2013·Biochimica Et Biophysica Acta·Masato IshigamiKazumitsu Ueda
Apr 13, 2013·Journal of Computer-aided Molecular Design·G Madhavi SastryWoody Sherman
Oct 25, 2013·Protein Science : a Publication of the Protein Society·Jingzhi LiStephen G Aller
May 2, 2014·Nucleic Acids Research·Marco BiasiniTorsten Schwede
May 8, 2014·Journal of Chemical Theory and Computation·Callum J DicksonRoss C Walker
Aug 19, 2014·Journal of Computational Chemistry·Emilia L WuWonpil Im
Nov 11, 2014·Nature·Janine D BrunnerRaimund Dutzler
Nov 18, 2015·Journal of Chemical Theory and Computation·James A MaierCarlos Simmerling
Jul 9, 2013·Journal of Chemical Theory and Computation·Daniel R Roe, Thomas E Cheatham
Aug 14, 2012·Journal of Chemical Theory and Computation·Devleena ShivakumarWoody Sherman
Sep 10, 2013·Journal of Chemical Theory and Computation·Romelia Salomon-FerrerRoss C Walker
Feb 8, 2011·Journal of Chemical Theory and Computation·Mats H M OlssonJan H Jensen
May 11, 2010·Journal of Chemical Theory and Computation·Devleena ShivakumarWoody Sherman
Mar 12, 2017·The Journal of Biological Chemistry·Suneet ShuklaSuresh V Ambudkar
Jun 13, 2017·Cell·Hongwu QianXin Gong
May 23, 2018·Nucleic Acids Research·Andrew WaterhouseTorsten Schwede
Dec 25, 2019·Nature Structural & Molecular Biology·Jeppe A OlsenKaspar P Locher

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.