Evidence for a dimeric structure of rat liver serine dehydratase

The International Journal of Biochemistry & Cell Biology
Hirofumi OgawaN H Huh

Abstract

Rat liver serine dehydratase (SDH) is known to be involved in gluconeogenesis. It has long been believed to be a dimeric protein with the subunit molecular weight (M(r)) of 34,000. Recently, sheep liver SDH was reported to be a monomer with a M(r) of 38,000. The native M(r) of rat SDH was only determined by the ultracentrifugation method more than three decades ago, and that of sheep SDH was done by the method of gel chromatography. The primary to quaternary structures of a given enzyme in a specific mammalian organ are usually conserved among various species. The aim of the present investigation is to clarify the structural differences between rat and sheep SDHs. First, we found that the amino acid composition reported for sheep SDH was statistically similar to that of rat SDH. Second, immunoblot analysis using anti-rat SDH IgG as the probe showed the size of sheep SDH to be a M(r) of 30,500, whereas that of SDH was about M(r) of 35,000. On the other hand, the native size of rat SDH was assessed by two methods: (1) the laser light scattering method demonstrated that rat SDH had a M(r) of 66,800, consistent with the previous value (M(r)=64,000); (2) cross-linking experiments of the purified rat SDH with dimethyl suberimidate re...Continue Reading

References

Nov 25, 1988·Nucleic Acids Research·H OgawaH C Pitot
Feb 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·H OgawaM Fujioka
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·H OgawaH C Pitot
Jul 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·G E Davies, G R Stark
Feb 16, 2000·Structure·G SchneiderY Lindqvist

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Citations

Aug 1, 2012·Journal of Proteome Research·Xiu-Ju ZhaoYulan Wang
Apr 4, 2006·Biochimica Et Biophysica Acta·Hirofumi OgawaMasashi Kobayashi
Oct 25, 2008·Biological Reviews of the Cambridge Philosophical Society·Erika M NowakGordon W Schuett
Nov 5, 2003·Biochemistry·Taro YamadaFusao Takusagawa

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