Evidence for a GTP-dependent increase in membrane permeability for calcium in NG108-15 microsomes

Biochemical and Biophysical Research Communications
T JeanC B Klee

Abstract

The effect of GTP on Ca2+ uptake and release was studied in a microsomal fraction isolated from neuroblastoma x glioma hybrid NG108-15 cells. GTP did not alter the ATP-dependent initial uptake of Ca2+ but markedly enhanced the efflux of Ca2+ from microsomes. GTP-dependent Ca2+ release requires the presence of millimolar concentration of Mg2+. The effect of GTP was not mimicked by other nucleotides and was competitively blocked by the thiophosphate analogue of GTP, GTP gamma S but not by the non-hydrolyzable nucleotide GMP-PNP. Addition of an inhibiting concentration of GTP gamma S after completion of GTP-induced calcium release did not result in a re-uptake of Ca2+, showing the irreversibility of the releasing effect of GTP. Our data are consistent with the hypothesis of Ca2+-dependent GTP-induced opening of a channel responsible for vectorial transport of Ca2+ ions from one intracellular compartment to another. A model is proposed suggesting that the GTP-binding protein is a GTP-specific diacylglycerol kinase.

References

Jan 1, 1987·Annual Review of Biochemistry·A G Gilman
Jan 1, 1987·Annual Review of Biochemistry·M J Berridge
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J M MullaneyD L Gill
May 15, 1987·The Biochemical Journal·A P DawsonJ G Comerford
Dec 1, 1985·The Biochemical Journal·C W Taylor, J W Putney

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Citations

Nov 1, 1992·Pharmacology & Therapeutics·L MissiaenR Casteels

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