PMID: 8448382Feb 1, 1993Paper

Evidence for a lactose-mediated association between two nuclear carbohydrate-binding proteins

Glycobiology
A P SèveJ Hubert

Abstract

Nuclear proteins were extracted in 2 M NaCl from membrane-depleted nuclei isolated from HL60 cells. Extracted proteins were submitted to affinity chromatography columns containing immobilized glucose, galactose or lactose. The polypeptides present in the different eluted fractions were resolved by SDS-PAGE and were either silver stained or analysed by immunoblotting with monoclonal or polyclonal antibodies, respectively, raised against the glucose-binding protein CBP67 and the galactose-binding proteins CBP35 and L14. The results presented here show that HL60 cell nuclei contain CBP35 and a glucose-binding lectin of 70 kDa (CBP70). These data account for the previously reported binding of neoglycoproteins containing glucosyl and galactosyl residues to HL60 cell nuclei. Furthermore, the present study provides the new information that CBP35 can associate with CBP70 by interactions dependent on the binding of CBP35 to lactose, and the results of some affinity chromatography experiments strongly suggest that CBP35 and CBP70 associate by protein-protein interactions. The potential function of this lactose-mediated interaction is discussed with respect to data recently reported by others showing that CBP35 is involved in in vitro mRN...Continue Reading

Citations

Sep 1, 1997·Journal of Cellular Biochemistry·C RousseauA P Sève
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Dec 1, 1994·Journal of Cellular Biochemistry·M FelinA P Sève
Apr 1, 1994·The International Journal of Biochemistry·H J Gabius
Aug 15, 1993·Mechanisms of Ageing and Development·G LaucH C Schröder
May 27, 2003·Biochimie·Joelle BottiChristian Derappe
Sep 12, 2002·Biochimica Et Biophysica Acta·Fu-Tong LiuJohn L Wang
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May 30, 2015·Colloids and Surfaces. B, Biointerfaces·Ali Dehshahri, Hossein Sadeghpour
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Dec 9, 2020·International Journal of Molecular Sciences·Valeria BlandaGiuliana Fortunato

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