PMID: 6403946Apr 1, 1983Paper

Evidence for a multiple random coupling mechanism in the alpha-ketoglutarate dehydrogenase multienzyme complex of Escherichia coli: a computer model analysis

Proceedings of the National Academy of Sciences of the United States of America
M L HackertL J Reed

Abstract

A computer modeling system was used to analyze experimental data for inactivation of the Escherichia coli alpha-ketoglutarate dehydrogenase complex accompanying release of lipoic acid residues by lipoamidase and by trypsin [Stepp, L. R., Bleile, D. M., McRorie, D. K., Pettit, F. H. & Reed, L. J. (1981) Biochemistry 20, 4555-4560]. The results provide insight into the active-site coupling mechanism in the alpha-ketoglutarate dehydrogenase complex. The model studies indicate that the overall activity of the alpha-ketoglutarate dehydrogenase complex is influenced by redundancies and random processes that we describe as a multiple random coupling mechanism. More than one lipoyl moiety services each E1 subunit (alpha-ketoglutarate dehydrogenase, EC 1.2.4.2), and an extensive lipoyl-lipoyl interaction network for exchange of electrons and possibly acyl groups must also be present. The best fit between computed and experimental data was obtained with a model that has two lipoyl moieties servicing each E1 subunit and a lipoyl-lipoyl interaction network that links all lipoyl moieties on the E2 cube (dihydrolipoamide succinyltransferase, EC 2.3.1.61). The single lipoyl moiety on an E2 subunit is assumed to service the coenzyme A-dependen...Continue Reading

References

Oct 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·J H Collins, L J Reed
Nov 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·M J DansonR N Perham
Jul 25, 1977·Biochemical and Biophysical Research Communications·D C SpeckhardP A Frey
Feb 15, 1979·European Journal of Biochemistry·G Hale, R N Perham
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·D M BleileL J Reed
Aug 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·L J ReedR M Oliver
Jun 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·D J DerosierL J Reed
May 14, 1980·Biochemical and Biophysical Research Communications·R H WhiteL J Reed

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Citations

Jul 10, 1998·Biochimica Et Biophysica Acta·A de KokA H Westphal
May 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M L HackertL J Reed
Feb 26, 2009·The Journal of Biological Chemistry·Chad A BrautigamDavid T Chuang
Feb 15, 2000·Annals of the New York Academy of Sciences·K F Sheu, J P Blass
Mar 28, 1986·Biochemical and Biophysical Research Communications·T WagenknechtD DeRosier

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