Evidence for a Na+-Ca2+ exchanger in rat pancreatic ducts

FEBS Letters
M J HugI Novak

Abstract

Only recently has it been recognized that intracellular Ca2+ is an important cellular mediator in pancreatic ducts. The aim of the present study was to characterize the Ca2+ efflux pathway in ducts freshly prepared from rat pancreas. Lowering of extracellular Na+ concentration resulted in a significant increase in intracellular Ca2+. This effect was fast, reversible, dependent on the extracellular Na+ concentration and did not correlate with intracellular pH changes. It was abolished in Ca2+-free solutions, indicating that the outwardly directed Na+ gradient was directly coupled to a flufenamate insensitive Ca2+ influx. Removal and reintroduction of extracellular Na+ induced transient hyperpolarization and depolarization of Vm, respectively. Taken together, our data indicate that pancreatic ducts possess an electrogenic Na+-Ca2+ exchanger, which under control conditions is responsible for transporting Ca2+ out of resting duct cells.

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Citations

May 5, 2004·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Nadja MøbjergIvana Novak
Jan 28, 1999·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Y FangL He
Oct 26, 2013·The Journal of Biological Chemistry·Andrew D JamesJason I E Bruce
Jun 23, 2004·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Agnes KittelJean Sévigny
Nov 8, 2002·Biochimica Et Biophysica Acta·Ivana Novak, Mette R Hansen

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