PMID: 8601690Apr 1, 1996Paper

Evidence for a nonlysosomal origin of the acrosome

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
J A Martínez-MenárguezJ Ballesta

Abstract

We studied the biogenesis of the acrosome in sperm cells in immunogold-labeled ultrathin cryosections of rat testis, using a variety of antibodies against endosomal/lysosomal marker protein and acrosin, the major secretory protein of sperm cells. As expected, acrosomes and proacrosomal vesicles in the trans-Golgi region contained abundant acrosin. Rat lysosomal membrane glycoprotein (lgp) 120 and mouse lysosome-associated membrane protein-1 were not detectable in the acrosomal membrane. Similarly, the late endosomal markers cation-dependent and -independent mannose 6-phosphate receptors were absent from the acrosome and proacrosomal vesicles. Therefore, acrosomes do not exhibit these endosomal/lysosomal features. Apart from (pro) acrosomal vesicles, both spermatocytes and spermatids contained classical lysosomes (positive for rat lgp 120, mouse lysosome-associated membrane protein-1, and cathepsin D) that were negative for acrosin. Quantitative analysis of the immunogold labeling showed that spermatocytes express more mannose 6-phosphate receptors and lgp 120 than spermatids, whereas the opposite situation existed for acrosin. These data indicate differential synthetic activity of lysosomal and acrosomal constituents in differe...Continue Reading

Citations

Jul 28, 1999·Molecular Reproduction and Development·K Huh, L S Yi
Jul 24, 2008·Histochemistry and Cell Biology·Guillermo Vivero-SalmerónJosé A Martínez-Menárguez
Nov 15, 2011·Histochemistry and Cell Biology·Keiichiro YogoTetsuya Kohsaka
Nov 7, 2000·Biochimica Et Biophysica Acta·F M Flesch, B M Gadella
Jan 18, 2016·Seminars in Cell & Developmental Biology·Giovanna Berruti
Jun 4, 2008·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Galder ValbuenaFrancisco José Sáez
Apr 28, 2004·Biochemical and Biophysical Research Communications·M BrahmarajuPradeep G Kumar
Mar 13, 2015·Cytoskeleton·Ling ZhangZhibing Zhang
Jun 20, 2001·Experimental Cell Research·J Ramalho-SantosG Schatten
Feb 10, 2012·Spermatogenesis·Giovanna Berruti, Chiara Paiardi
Aug 4, 2016·Autophagy·D J SidjaninWilliam T Jackson
Sep 26, 2002·Biology of Reproduction·João Ramalho-SantosRicardo D Moreno
Apr 30, 2017·Molecular Reproduction and Development·Bart M Gadella
May 20, 2003·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Peter RedeckerTobias M Boeckers
Aug 9, 2006·Molecular Reproduction and Development·Ricardo D Moreno, Carlos P Alvarado
Oct 18, 2019·Frontiers in Cell and Developmental Biology·Muhammad Babar KhawarWei Li

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