Evidence for a potent lipid secretagogue in the cyst fluids of patients with autosomal dominant polycystic kidney disease.

Journal of the American Society of Nephrology : JASN
J J GranthamM Sharma

Abstract

Transepithelial fluid secretion appears to be an important factor in the progressive enlargement of cysts in autosomal dominant polycystic kidney disease. Evidence indicates that the fluid within cysts harbors an autocrine, paracrine, or endocrine secretagogue with the capacity to modulate the rate of cyst expansion. Fluids from five patients with autosomal dominant polycystic kidney disease were studied to determine the chemical nature and the physiologic function of the putative secretagogue. The secretory activity of cyst fluid assayed with polarized monolayers of Madin Darby canine kidney cells could be ascribed to a lipophilic substance of molecular weight < 3,500 d that was not destroyed by freezing, boiling, or proteolytic digestion. This lipid stimulated the production of intracellular cAMP and increased the rate of fluid secretion when added to either surface of cultured renal epithelial cells. Anion exchange chromatography revealed biologic secretory activity to a greater extent in the neutral lipid than in the fatty acid and phospholipid fractions separated from cyst fluid. More extensive chromatographic separation showed preferential appearance of the secretagogue in a fraction of neutral lipids enriched in monoglyc...Continue Reading

Citations

Dec 11, 2002·Renal Failure·Rebecca Backenroth, Mordecai M Popovtzer
Apr 19, 2008·Journal of Proteome Research·Esmé WaandersJoost P H Drenth
Jan 9, 2004·The New England Journal of Medicine·Patricia D Wilson
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Jan 25, 2011·Biochimica Et Biophysica Acta·Sara TerrynOlivier Devuyst
Jun 7, 2011·Acta Pharmacologica Sinica·Ying SunBao-xue Yang

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