Evidence for a pro-apoptotic phenotype in skeletal muscle of hypertensive rats

Biochemical and Biophysical Research Communications
Joe Quadrilatero, James W E Rush

Abstract

In this report, we demonstrate that soleus muscle of spontaneously hypertensive rats (SHR) had significantly lower protein levels of apoptosis repressor with caspase recruitment domain (ARC) and X-linked inhibitor of apoptosis protein (XIAP) as well as significantly higher protein levels of second mitochondria-derived activator of caspase (Smac) and procaspase-8 compared to normotensive Wistar-Kyoto (WKY) rats. In addition, soleus muscle from hypertensive rats had significantly increased caspase-8 proteolytic enzyme activity as well as significantly elevated reactive oxygen species (ROS) generation and higher hydrogen peroxide (H(2)O(2)) content. There was no change in the protein levels of the antioxidant enzymes, catalase, copper-zinc superoxide dismutase (CuZnSOD), and manganese superoxide dismutase (MnSOD). Interestingly, ARC protein migrated at approximately 32kDa in SHR but at 30kDa in WKY rat muscle; possibly indicating a post-translational modification. These results demonstrate that soleus muscle of hypertensive rats display a pro-apoptotic phenotype and augmented ROS generation.

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Citations

Jun 24, 2011·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Yanlei HaoStephen E Alway
May 5, 2011·The Journal of Physiology·Bee T TengParco M Siu
Dec 23, 2009·Biochemical and Biophysical Research Communications·Joe Quadrilatero, Darin Bloemberg
Dec 7, 2010·Journal of Cellular and Molecular Medicine·Agnieszka H Ludwig-GalezowskaMarkus Rehm
Oct 15, 2013·Acta Physiologica·D BloembergJ Quadrilatero
Dec 10, 2009·American Journal of Physiology. Endocrinology and Metabolism·Joe QuadrilateroLawrence L Spriet
Dec 4, 2019·Oncology Letters·Zhongjie YuPeifeng Li

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